Masuho Y, Matsumoto Y, Sugano T, Fujinaga S, Minamishima Y
J Gen Virol. 1987 May;68 ( Pt 5):1457-61. doi: 10.1099/0022-1317-68-5-1457.
Hybridomas producing human monoclonal antibodies (MAbs) against human cytomegalovirus (CMV) were generated by fusion of human spleen cells and mouse myeloma cells. Two of the six MAbs obtained neutralized viral infectivity even at concentrations lower than 1 microgram/ml. One MAb required complement for neutralization but the other did not. Both MAbs recognized viral proteins of Mr 130,000 and 55,000. Furthermore, these neutralizing MAbs bound to the surface membrane of CMV-infected cells. These results suggest that human MAbs may provide a new means of passive immunization against CMV infection in humans.
通过人脾细胞与小鼠骨髓瘤细胞融合，产生了针对人巨细胞病毒（CMV）的人单克隆抗体（MAb）杂交瘤。所获得的6种MAb中有2种即使在浓度低于1微克/毫升时也能中和病毒感染性。一种MAb中和需要补体，而另一种则不需要。两种MAb都识别分子量为130,000和55,000的病毒蛋白。此外，这些中和性MAb与CMV感染细胞的表面膜结合。这些结果表明，人MAb可能为人类被动免疫预防CMV感染提供一种新方法。
