Pourcho R G, Goebel D J
J Neurosci. 1987 Apr;7(4):1178-88. doi: 10.1523/JNEUROSCI.07-04-01178.1987.
Morphological subclasses of cone bipolar cells in the cat retina were studied in Golgi preparations and through the combination of Golgi impregnation with quantitative electron-microscopic autoradiography of 3H-glycine accumulation. Camera lucida drawings, computer-assisted rotations, and radial sections provided the morphological details used in assigning individual cells to morphologically defined subclasses. Selected Golgi-impregnated cells from retinas preloaded with 3H-glycine were sectioned for autoradiographic analysis. Grain densities were quantitated and compared on a normalized scale with the labeling of amacrine cells with 3H-glycine. Eleven types of cone bipolar cells were identified, 6 with axon terminals ramifying in sublamina a and 5 with axons terminating in sublamina b. Of these cells, types CBb2 and CBb5 showed the highest affinity for glycine, with labeling at 41 and 38% with respect to the most heavily labeled amacrine cells. Types CBb1, CBb3, CBa2, and CBa6 were labeled at approximately 20% of maximal labeling. The accumulation of glycine by these cells suggests that they may be involved in providing inhibitory input to the inner retina.
利用高尔基染色法，并通过将高尔基浸染与3H-甘氨酸积累的定量电子显微镜放射自显影相结合的方法，对猫视网膜中视锥双极细胞的形态学亚类进行了研究。通过摄像描绘器绘图、计算机辅助旋转和径向切片，提供了用于将单个细胞归为形态学定义亚类的形态学细节。对预先用3H-甘氨酸加载的视网膜中选定的高尔基浸染细胞进行切片，用于放射自显影分析。对颗粒密度进行定量，并在标准化尺度上与用3H-甘氨酸标记的无长突细胞的标记进行比较。鉴定出11种视锥双极细胞类型，其中6种的轴突终末在a亚层分支，5种的轴突终末在b亚层。在这些细胞中，CBb2型和CBb5型对甘氨酸的亲和力最高，相对于标记最重的无长突细胞，标记率分别为41%和38%。CBb1型、CBb3型、CBa2型和CBa6型的标记率约为最大标记率的20%。这些细胞对甘氨酸的积累表明，它们可能参与向内视网膜提供抑制性输入。
