Kolb H, Cuenca N, Dekorver L
Physiology Department, University of Utah School of Medicine, Salt Lake City 84108.
J Comp Neurol. 1991 Aug 8;310(2):267-84. doi: 10.1002/cne.903100210.
Postembedding electron microscope immunocytochemistry of glycine and GABA conjugated to colloidal gold has been applied to pre-embedded cat retina stained with the antibody against tyrosine hydroxylase (Toh+). Toh+ stained cells are the equivalent of A18 amacrine cells of Golgi descriptions (Kolb et al., '81). The dendrites of Toh+ cells synapse upon several different types of glycine-positive amacrine cell bodies. We suggest that these are the A8, A3/A4, and AII amacrine cell varieties by analogous immunocytochemical staining intensity, to glycine autoradiographic labeling intensity (Pourcho and Goebel, '85). The greatest number of synapses from Toh+ dendrites are directed at the least glycine-positive amacrine, which is the AII cell by all morphological criteria. A few glycine-positive profiles are also presynapatic to the Toh+ stained cell body itself. Toh+ profiles are also presynaptic to GABA-positive amacrine cell bodies. The commonest amacrine synapsed upon is very heavily labeled with GABA immunocytochemistry. We consider it to be the A17 amacrine cell, which is known to label strongly by [3H] muscimol autoradiography (Pourcho and Goebel, '83). The cell body of the Toh+ amacrine cell also receives many synapses, which appear to be GABA-positive, and Toh+ profiles running in stratum 1 of the inner plexiform layer (IPL) are both pre- and postsynaptic to GABA-positive amacrine cell profiles. In addition, the cell body and primary dendrites of the Toh+ cell receive input from a bipolar type and GABA- or glycine-negative profiles. GABA-positive profiles, belonging to the interplexiform cell (IPC), are synapsed upon by Toh+ profiles that run in the outer plexiform layer (OPL).(ABSTRACT TRUNCATED AT 250 WORDS)
将与胶体金偶联的甘氨酸和γ-氨基丁酸进行包埋后电子显微镜免疫细胞化学，已应用于用抗酪氨酸羟化酶抗体（Toh+）染色的预包埋猫视网膜。Toh+染色的细胞相当于高尔基体描述中的A18无长突细胞（Kolb等人，1981年）。Toh+细胞的树突与几种不同类型的甘氨酸阳性无长突细胞体形成突触。根据类似的免疫细胞化学染色强度与甘氨酸放射自显影标记强度（Pourcho和Goebel，1985年），我们认为这些是A8、A3/A4和AII无长突细胞类型。来自Toh+树突的突触数量最多的是甘氨酸阳性最少的无长突细胞，根据所有形态学标准，这是AII细胞。一些甘氨酸阳性的形态也与Toh+染色的细胞体本身形成突触前联系。Toh+形态也与γ-氨基丁酸阳性无长突细胞体形成突触前联系。与之形成突触的最常见无长突细胞被γ-氨基丁酸免疫细胞化学强烈标记。我们认为它是A17无长突细胞，已知其通过[3H]蝇蕈醇放射自显影强烈标记（Pourcho和Goebel，1983年）。Toh+无长突细胞的细胞体也接受许多似乎是γ-氨基丁酸阳性的突触，并且在内网状层（IPL）第1层中运行的Toh+形态与γ-氨基丁酸阳性无长突细胞形态既有突触前联系又有突触后联系。此外，Toh+细胞的细胞体和初级树突接受来自一种双极类型以及γ-氨基丁酸或甘氨酸阴性形态的输入。属于网间细胞（IPC）的γ-氨基丁酸阳性形态与在外网状层（OPL）中运行的Toh+形态形成突触。（摘要截断于250字）
