Zhao Shankun, Liu Luhao, Kang Ran, Li Futian, Li Ermao, Zhang Tao, Luo Jintai, Zhao Zhigang
Department of Urology & Andrology, Minimally Invasive Surgery Center, Guangdong Provincial Key Laboratory of Urology, The First Affiliated Hospital of GuangZhou Medical University, Guangzhou, Guangdong, China.
Department of Urology & Andrology, Minimally Invasive Surgery Center, Guangdong Provincial Key Laboratory of Urology, The First Affiliated Hospital of GuangZhou Medical University, Guangzhou, Guangdong, China.
Urology. 2016 May;91:243.e7-243.e12. doi: 10.1016/j.urology.2016.02.021. Epub 2016 Feb 23.
To evaluate the effects of Shengjing capsule on erectile function in a castrated rat model, and further to investigate the underlying molecular mechanism.
Thirty male Sprague-Dawley rats were randomly divided into six groups (n = 5 per group), including sham group, castration group, testosterone replacement group, high-dose Shengjing capsule group, medium-dose Shengjing capsule group, and low-dose Shengjing capsule group. The weight of the body and androgen-sensitive organs, and the serum level of testosterone were assessed. Erectile function was evaluated using cavernous nerve electrical stimulation after treatment. Corpus cavernosum tissue was examined by Masson's trichrome staining, immunohistochemistry, quantitative reverse transcriptase-polymerase chain reaction, and Western blot.
Serum testosterone level, mean weights of body, and accessory sexual organs were not significantly different between Shengjing capsule treatment and the castration groups (P > .05 for all). Significant recovery of erectile function and the increased smooth muscle components were observed in the Shengjing capsule treatment group as compared with the castration group (P < .01 and P < .01, respectively). The gene and protein expression of 3 subtypes of nitric oxide synthase (NOS)-neuronal (nNOS), inducible (iNOS), and endothelial (eNOS) -in cavernous tissue in Shengjing capsule-treated rats were significantly higher than in the castration group (P < .05 for all). Phosphodiesterase type 5 messenger ribonucleic acid and protein expression in each group followed a trend similar to that of smooth muscle content.
These results show that Shengjing capsule improves the erectile function by protecting the smooth muscle content and enhancing NOS activity in penile tissues of castrated male rats.
评估生精胶囊对去势大鼠模型勃起功能的影响，并进一步探究其潜在的分子机制。
将30只雄性Sprague-Dawley大鼠随机分为6组（每组n = 5），包括假手术组、去势组、睾酮替代组、生精胶囊高剂量组、生精胶囊中剂量组和生精胶囊低剂量组。评估体重、雄激素敏感器官以及血清睾酮水平。治疗后采用海绵体神经电刺激评估勃起功能。通过Masson三色染色、免疫组织化学、定量逆转录聚合酶链反应和蛋白质免疫印迹法检测海绵体组织。
生精胶囊治疗组与去势组之间血清睾酮水平、平均体重和附属性器官重量无显著差异（均P > 0.05）。与去势组相比，生精胶囊治疗组勃起功能显著恢复，平滑肌成分增加（分别为P < 0.01和P < 0.01）。生精胶囊治疗大鼠海绵体组织中3种一氧化氮合酶（NOS）亚型——神经元型（nNOS）、诱导型（iNOS）和内皮型（eNOS）的基因和蛋白表达显著高于去势组（均P < 0.05）。各组5型磷酸二酯酶信使核糖核酸和蛋白表达趋势与平滑肌含量相似。
这些结果表明，生精胶囊通过保护平滑肌含量和增强去势雄性大鼠阴茎组织中的NOS活性来改善勃起功能。
