利用逆转录病毒表达载体进行cDNA克隆：pp60c-src cDNA克隆的产生
cDNA cloning with a retrovirus expression vector: generation of a pp60c-src cDNA clone.
作者信息
Kaplan P L, Simon S, Cartwright C A, Eckhart W
出版信息
J Virol. 1987 May;61(5):1731-4. doi: 10.1128/JVI.61.5.1731-1734.1987.
Abstract
We used a murine retroviral expression vector, containing a genomic clone of the chicken c-src gene, a bacterial origin of replication, and a selectable marker, to remove 10 introns from the c-src gene. All 10 introns were removed accurately, and no mutations were introduced. The processed gene encoded a functional pp60c-src protein tyrosine kinase.
摘要
我们使用了一种鼠逆转录病毒表达载体，其包含鸡c-src基因的基因组克隆、细菌复制起点和一个选择标记，以从c-src基因中去除10个内含子。所有10个内含子均被准确去除，且未引入突变。加工后的基因编码一种功能性的pp60c-src蛋白酪氨酸激酶。
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本文引用的文献
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Overexpression of the c-src protein does not induce transformation of NIH 3T3 cells.c-src蛋白的过表达不会诱导NIH 3T3细胞发生转化。
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