在 Raman 光谱学中，通过在石墨烯模板上简便合成金纳米六边形，用于生物传感癌症和癌症干细胞。

Facile synthesis of gold nanohexagons on graphene templates in Raman spectroscopy for biosensing cancer and cancer stem cells.

机构信息

Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan.

Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Department of Chemistry, Assuit University, Assuit 71515, Egypt.

出版信息

Biosens Bioelectron. 2014 May 15;55:180-6. doi: 10.1016/j.bios.2013.11.037. Epub 2013 Nov 21.

DOI:10.1016/j.bios.2013.11.037

PMID:24374301

Abstract

Several surface enhanced Raman spectroscopy (SERS) substrates were prepared based on in situ nucleation of gold nanohexagons (Au) on graphene (G) nanosheets (Au@G), G, Au nanoparticles and Au conjugated G nanomaterials. These were applied to enhance Raman scattering and to differentiate human breast normal, cancer and cancer stem cells. These SERS substrates at concentrations of 100 µg/1 × 10(4) cells led to 5.4 fold increase in detecting breast cancer cells (BCCs) and 4.8 fold of sensitivity for detecting breast cancer stem cells (BCSCs) and they were able to identify and differentiate between normal cells, cancer cells and cancer stem cells. These approaches are rapid, simple and reliable for healthy normal cells, cancer cells and cancer stem cell detection which have a huge potential for cancer research for medical or biomedicine applications.

摘要

几种基于金纳米六棱柱（Au）在石墨烯（G）纳米片（Au@G）、G、金纳米粒子和 Au 共轭 G 纳米材料上原位成核的表面增强拉曼光谱（SERS）基底被制备出来。这些基底被用于增强拉曼散射，并区分人乳腺癌正常细胞、癌细胞和癌症干细胞。这些 SERS 基底在浓度为 100 µg/1 × 10(4)细胞时，导致乳腺癌细胞（BCCs）的检测灵敏度提高了 5.4 倍，乳腺癌干细胞（BCSCs）的检测灵敏度提高了 4.8 倍，并且能够识别和区分正常细胞、癌细胞和癌症干细胞。这些方法对于健康正常细胞、癌细胞和癌症干细胞的检测快速、简单和可靠，对于癌症研究和医学或生物医学应用具有巨大的潜力。