Terpilowska Sylwia, Gluszek Stanislaw, Czerwosz Elzbieta, Wronka Halina, Firek Piotr, Szmidt Jan, Suchanska Malgorzata, Keczkowska Justyna, Kaczmarska Bozena, Kozlowski Mirosław, Diduszko Ryszard
Jan Kochanowski University, Collegium Medicum, Department of Surgical Medicine with the Laboratory of Medical Genetics, IX Wieków Kielc 19A Av., 25-317 Kielce, Poland.
Institute of Micro- and OptoElectronics, Warsaw Technical University, Nowowiejska 15/19, 00-665 Warszawa, Poland.
Materials (Basel). 2022 Aug 24;15(17):5826. doi: 10.3390/ma15175826.
The application of nano-Ag grains as antiviral and antibacterial materials is widely known since ancient times. The problem is the toxicity of the bulk or big-size grain materials. It is known that nano-sized silver grains affect human and animal cells in some medical treatments. The aim of this study is to investigate the influence of nano-Ag grains embedded in a carbonaceous matrix on cytotoxicity, genotoxicity in fibroblasts, and mutagenicity. The nanocomposite film is composed of silver nanograins embedded in a carbonaceous matrix and it was obtained via the PVD method by deposition from two separated sources of fullerenes and silver acetate powders. This method allows for the preparation of material in the form of a film or powder, in which Ag nanograins are stabilized by a carbon network. The structure and morphology of this material were studied using SEM/EDX, XRD, and Raman spectroscopy. The toxicology studies were performed for various types of the material differing in the size of Ag nanograins. Furthermore, it was found that these properties, such as cell viability, genotoxicity, and mutagenicity, depend on Ag grain size.
纳米银颗粒作为抗病毒和抗菌材料的应用自古以来就广为人知。问题在于块状或大尺寸颗粒材料的毒性。众所周知，在某些医学治疗中，纳米尺寸的银颗粒会影响人类和动物细胞。本研究的目的是研究嵌入碳质基质中的纳米银颗粒对细胞毒性、成纤维细胞中的遗传毒性和致突变性的影响。该纳米复合薄膜由嵌入碳质基质中的银纳米颗粒组成，它是通过物理气相沉积（PVD）法，从富勒烯和醋酸银粉末这两种分离的源材料沉积而获得的。这种方法允许制备薄膜或粉末形式的材料，其中银纳米颗粒通过碳网络得以稳定。使用扫描电子显微镜/能谱仪（SEM/EDX）、X射线衍射仪（XRD）和拉曼光谱对该材料的结构和形态进行了研究。针对银纳米颗粒尺寸不同的各种类型材料进行了毒理学研究。此外，还发现这些性质，如细胞活力、遗传毒性和致突变性，取决于银颗粒的大小。
