Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan; Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan.
Laboratory of Oral Chronobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan; Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Yamadaoka, Suita, Osaka 565-0871, Japan.
FEBS Lett. 2014 Jan 31;588(3):459-65. doi: 10.1016/j.febslet.2013.12.007. Epub 2013 Dec 25.
The emergence of the circadian rhythm is a dramatic and physiologically essential event for mammals to adapt to daily environmental cycles. It has been demonstrated that circadian rhythms develop during the embryonic stage even when the maternal central pacemaker suprachiasmatic nucleus has been disrupted. However, the mechanisms controlling development of the circadian clock are not yet fully understood. Here, we show that the circadian molecular oscillation in primary dispersed embryonic cells and explanted salivary glands obtained from mPER2(Luc) mice embryos developed cell- or tissue-autonomously even in tissue culture conditions. Moreover, the circadian clock in the primary mPER2(Lu)(c) fibroblasts could be reprogrammed by the expression of the reprogramming factors. These findings suggest that mammalian circadian clock development may interact with cellular differentiation mechanisms.
昼夜节律的出现是哺乳动物适应每日环境周期的一个戏剧性的、生理上至关重要的事件。已经证明，即使破坏了母体中央起搏器视交叉上核，昼夜节律也会在胚胎阶段发展。然而，控制生物钟发育的机制尚未完全了解。在这里，我们表明，从 mPER2（Luc）小鼠胚胎获得的原代分散胚胎细胞和离体唾液腺中的昼夜分子振荡即使在组织培养条件下也能自主地进行细胞或组织自主发展。此外，通过表达重编程因子，原代 mPER2（Lu）（c）成纤维细胞中的生物钟可以被重新编程。这些发现表明，哺乳动物生物钟的发育可能与细胞分化机制相互作用。
