State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Department of Chemistry, Nanchang University, Nanchang 330031, China.
Department of Chemistry, Nanchang University, Nanchang 330031, China.
J Hazard Mater. 2014 Feb 15;266:60-7. doi: 10.1016/j.jhazmat.2013.12.006. Epub 2013 Dec 13.
Phenols are well known noxious compounds, which are often found in various water sources. A novel analytical method has been researched and developed based on the properties of hemin-graphene hybrid nanosheets (H-GNs). These nanosheets were synthesized using a wet-chemical method, and they have peroxidase-like activity. Also, in the presence of H2O2, the nanosheets are efficient catalysts for the oxidation of the substrate, 4-aminoantipine (4-AP), and the phenols. The products of such an oxidation reaction are the colored quinone-imines (benzodiazepines). Importantly, these products enabled the differentiation of the three common phenols - pyrocatechol, resorcin and hydroquinone, with the use of a novel, spectroscopic method, which was developed for the simultaneous determination of the above three analytes. This spectroscopic method produced linear calibrations for the pyrocatechol (0.4-4.0 mg L(-1)), resorcin (0.2-2.0 mg L(-1)) and hydroquinone (0.8-8.0 mg L(-1)) analytes. In addition, kinetic and spectral data, obtained from the formation of the colored benzodiazepines, were used to establish multi-variate calibrations for the prediction of the three phenol analytes found in various kinds of water; partial least squares (PLS), principal component regression (PCR) and artificial neural network (ANN) models were used and the PLS model performed best.
酚类是众所周知的有害化合物，经常存在于各种水源中。本研究基于血红素-石墨烯杂化纳米片（H-GNs）的特性，开发了一种新的分析方法。这些纳米片是通过湿化学方法合成的，具有过氧化物酶样活性。此外，在 H2O2 存在的情况下，纳米片是 4-氨基安替比林（4-AP）和酚类底物氧化的有效催化剂。这种氧化反应的产物是有色的醌亚胺（苯并二氮杂）。重要的是，这些产物使三种常见的酚类物质——邻苯二酚、间苯二酚和对苯二酚能够通过一种新的、光谱方法来区分，该方法是为同时测定上述三种分析物而开发的。该光谱方法对邻苯二酚（0.4-4.0 mg L(-1))、间苯二酚（0.2-2.0 mg L(-1))和对苯二酚（0.8-8.0 mg L(-1))分析物产生了线性校准。此外，从有色苯并二氮杂形成获得的动力学和光谱数据用于建立多元校准，以预测各种水样中的三种酚类分析物；使用了偏最小二乘法（PLS）、主成分回归（PCR）和人工神经网络（ANN）模型，其中 PLS 模型表现最好。
