胃肠道中脂质过氧化氢衍生的蛋白质修饰。

Lipid hydroperoxide-derived modification of proteins in gastrointestinal tract.

作者信息

Naito Yuji, Takagi Tomohisa, Handa Osamu, Yoshikawa Toshikazu

机构信息

Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, 465 Kajiicho, Kamigyo-ku, Kyoto, 602-8566, Japan,

出版信息

Subcell Biochem. 2014;77:137-48. doi: 10.1007/978-94-007-7920-4_12.

DOI:10.1007/978-94-007-7920-4_12

PMID:24374925

Abstract

Role of lipid peroxidation in the pathogenesis of gastrointestinal diseases has been evaluated by measuring the tissue levels of lipid peroxides as thiobarbituric acid-reactive substances in the animal models as well as human. Recently, N (ε)-(hexanoyl)lysine (HEL) and 4-hydroxy-2-nonenal (HNE) are recognized as reliable and sensitive biomarkers for the early phase and the late phase of lipid peroxidation, respectively. The presence of HNE- and HEL-modified proteins has been demonstrated in in vivo models of several gastrointestinal diseases. In the present review, we introduced HNE-modification of TRPV1 channel in esophageal epithelial cells, HEL-modification of tropomyosin 1 (TMP1) in gastric cancer cells, and HEL-modification of gastrokine 1 in the healing of gastric ulcer.

摘要

脂质过氧化在胃肠道疾病发病机制中的作用已通过在动物模型和人体中测量脂质过氧化物作为硫代巴比妥酸反应性物质的组织水平来评估。最近，N（ε）-（己酰基）赖氨酸（HEL）和4-羟基-2-壬烯醛（HNE）分别被认为是脂质过氧化早期和晚期可靠且敏感的生物标志物。在几种胃肠道疾病的体内模型中已证实存在HNE和HEL修饰的蛋白质。在本综述中，我们介绍了食管上皮细胞中TRPV1通道的HNE修饰、胃癌细胞中肌动蛋白原肌球蛋白1（TMP1）的HEL修饰以及胃溃疡愈合过程中胃动素1的HEL修饰。