Minato Ken-ichiro, Miyake Yoshiaki
Department of Applied Biological Chemistry, Meijo University, 1-501 Shiogamaguchi, Nagoya, 468-8502, Japan,
Subcell Biochem. 2014;77:163-74. doi: 10.1007/978-94-007-7920-4_14.
We could proposed that N(ε)-(hexanoy)lysine, HEL, become a useful biomarker for detection of oxidative stress damage occurred by exhaustive exercise. We examined the preventive effect of flavonoid compound, eriocitrin, against exercise-induced oxidative damage in rat liver. Eriocitrin administration prior to exercise significantly suppressed the increases in thiobarbituric acid-reactive substance caused by lipid peroxidation during exhaustive exercise. The increase in the contents of HEL in rat liver was also abolished by eriocitrin administration. The concentration of oxidized glutathione was significantly increased by exercise, but the eriocitrin administration suppressed this increase. These results suggested that eriocitrin administration prior to exercise prevented oxidative damages caused by exhaustive exercise-induced oxidative stress. Therefore, it was suggested that HEL could be a good biomarker for oxidative stress, especially at earlier stage when oxidative damage was occurred by lipid peroxidation than a stage of harmful aldehyde formation. Moreover, it was suggested that eriocitrin metabolites, eriodictyol and 3, 4 - dihydroxyhydrocinnamic, might scavenge free radicals and reactive oxygen species, resulting in suppression of lipid peroxidation and reactive proteins with radicals to form HEL. These findings implied that eriocitrin might be useful as an anti-oxidative compound to protect oxidative stress damages.
我们可以提出，N(ε)-(己酰基)赖氨酸（HEL）可成为检测力竭运动所致氧化应激损伤的有用生物标志物。我们研究了类黄酮化合物圣草次苷对大鼠肝脏运动诱导的氧化损伤的预防作用。运动前给予圣草次苷可显著抑制力竭运动期间脂质过氧化引起的硫代巴比妥酸反应性物质的增加。给予圣草次苷也可消除大鼠肝脏中HEL含量的增加。运动可使氧化型谷胱甘肽浓度显著升高，但给予圣草次苷可抑制这种升高。这些结果表明，运动前给予圣草次苷可预防力竭运动诱导的氧化应激所致的氧化损伤。因此，提示HEL可能是氧化应激的良好生物标志物，尤其是在脂质过氧化导致氧化损伤的早期阶段，而不是有害醛形成的阶段。此外，提示圣草次苷的代谢产物圣草酚和3,4-二羟基氢化肉桂酸可能清除自由基和活性氧，从而抑制脂质过氧化以及与自由基反应的蛋白质形成HEL。这些发现表明，圣草次苷可能作为一种抗氧化化合物用于保护氧化应激损伤。
