Minato Ken-ichiro
Department of Applied Biological Chemistry, Meijo University, 1-501 Shiogamaguchi, Nagoya, 468-8502, Japan,
Subcell Biochem. 2014;77:73-82. doi: 10.1007/978-94-007-7920-4_6.
N(ε)-(hexanoyl)lysine (HEL) is known to be an oxidative lipid-decomposition product, and a powerful marker indicating oxidative stress in animal tissue. We investigated whether HEL could be useful as a marker in rice seeds damaged by oxidative stress during storage, as well as animals. We could show an accumulation of HEL in rice stored at high temperature (40 °C). This result significantly corresponded with an accumulation of TBARS. Rice germination deteriorated with non-enzymatic lipid peroxidation during storage at high temperature for a few months. These results suggested that a deterioration of rice germination ability resulted from oxidative damage caused by lipid peroxidation during storage. Moreover, HEL could become a useful marker for oxidative stress induced by lipid peroxidation. In addition, the activities of antioxidant enzymes, catalase and superoxide dismutase, significantly decreased in the rice seeds during storage at 40 °C. The relationship between accumulation of HEL and increases in antioxidant enzymes activities must be further studied. But, these results suggest that HEL might be a useful marker of oxidative stress in rice as well as in animals.
N(ε)-(己酰基)赖氨酸（HEL）是一种氧化脂质分解产物，也是指示动物组织氧化应激的有力标志物。我们研究了HEL是否可用作储存期间受氧化应激损伤的水稻种子以及动物体内的标志物。我们发现高温（40℃）储存的水稻中HEL会积累。这一结果与丙二醛（TBARS）的积累显著相关。在高温下储存几个月期间，水稻发芽率因非酶促脂质过氧化而降低。这些结果表明，水稻发芽能力的下降是储存期间脂质过氧化引起的氧化损伤所致。此外，HEL可能成为脂质过氧化诱导的氧化应激的有用标志物。另外，在40℃储存期间，水稻种子中的抗氧化酶过氧化氢酶和超氧化物歧化酶的活性显著降低。HEL积累与抗氧化酶活性增加之间的关系还有待进一步研究。但是，这些结果表明HEL可能是水稻以及动物体内氧化应激的有用标志物。
