Laboratoire d'Immunogénétique Moléculaire, EA 3034, Faculté de Médecine Purpan, Université Paul Sabatier, Toulouse 3, CHU de Toulouse, Toulouse, France.
Immunogenetics. 2014 Feb;66(2):105-13. doi: 10.1007/s00251-013-0752-3. Epub 2013 Dec 28.
The impact of feto-maternal histocompatibility on reproduction has inspired long-lasting debates. However, after the review of numerous articles, the impact of HLA allele sharing within couples on fecundity remains questionable. We decided to explore the impact of major histocompatibility complex (MHC) feto-maternal compatibility on reproduction in a cynomolgus macaque facility composed of animals of Mauritian descent. The Mauritian-derived macaque population presents a very restricted MHC polymorphism (only seven founding haplotypes) due to a strong founding bottleneck effect. The MHC polymorphism was investigated in 237 trios (male, female and offspring) using 17 microsatellite markers distributed across the MHC. Haplotypes were confirmed by segregation analysis. We evaluated the relative frequencies of MHC-compatible and MHC-semi-compatible offspring with the mothers. Among the 237 trios, we selected 42 trios for which the identity of the father is certain and for which the theoretical probabilities of fully compatible and semi-compatible offspring were equal. We found 11 offspring fully compatible and 31 offspring semi-compatible with their respective mother. The observed proportions were clearly outside the interval of confidence of 99 % and therefore most probably resulted from a selection of the semi-compatible offspring during pregnancy. We concluded that MHC fully compatible cynomolgus macaque offspring have a selective survival disadvantage in comparison with offspring inheriting a paternal MHC haplotype differing from maternal haplotypes.
胎儿-母体组织相容性对生殖的影响激发了持久的争论。然而，在回顾了大量文章之后，夫妻之间 HLA 等位基因共享对生育能力的影响仍然存在疑问。我们决定在一个由毛里求斯血统的动物组成的食蟹猴设施中探索主要组织相容性复合体 (MHC) 胎儿-母体相容性对生殖的影响。由于强烈的奠基者瓶颈效应，毛里求斯衍生的猕猴种群呈现出非常有限的 MHC 多态性（仅七个创始单倍型）。使用分布在 MHC 上的 17 个微卫星标记，在 237 个三（雄性、雌性和后代）中研究了 MHC 多态性。通过分离分析证实了单倍型。我们评估了与母亲具有 MHC 相容性和 MHC 半相容性后代的相对频率。在 237 个三家中，我们选择了 42 个三，其中父亲的身份是确定的，并且完全相容和半相容后代的理论概率相等。我们发现 11 个后代与各自的母亲完全相容，31 个后代与母亲半相容。观察到的比例明显超出了 99%置信区间，因此很可能是由于在怀孕期间选择了半相容后代。我们得出结论，与遗传自母系和父系单倍型不同的 MHC 完全相容的食蟹猴后代在选择性生存方面存在劣势。
