Pyziak L, Stasikowska-Kanicka O, Danilewicz M, Wągrowska-Danilewicz M
Łukasz Pyziak, Department of Nephropathology, Medical University of Lodz, Czechosłowacka 8/10, 92-216 Lodz, Poland, tel. +48 42 675 76 22, fax +48 42 679 01 91, e-mail:
Pol J Pathol. 2013 Dec;64(4):276-80. doi: 10.5114/pjp.2013.39336.
The aim of the study was to evaluate mast cell concentration and microvessel density in perilesional and intralesional regions of oral squamous cell carcinoma (OSCC) and furthermore to assess the possible relationship between the above-mentioned parameters. Paraffin-embedded specimens from 47 cases of OSCC and 12 cases of normal mucosa were investigated immunohistochemically with anti-CD-31 antibody to stain microvessels and anti-tryptase antibody to visualize mast cells. The degree of vascularization and mast cell infiltration was measured with an image analysis system. The study revealed considerably increased microvessel density and mast cell abundance in intralesional and perilesional regions of OSCCs in comparison to normal mucosa. There was a significant positive correlation between microvessel density and mast cell concentration in both localizations of OSCCs (p < 0.02, p < 0.001, respectively), whereas a comparable correlation was not observed in normal mucosa. The obtained results suggest that mast cells play an important role in the regulation of angiogenesis in OSCC, although there are aspects of their activity of potential diagnostic and therapeutic significance which require further research.
本研究旨在评估口腔鳞状细胞癌（OSCC）病灶周围和病灶内区域的肥大细胞浓度和微血管密度，并进一步评估上述参数之间的可能关系。对47例OSCC病例和12例正常黏膜的石蜡包埋标本进行免疫组织化学研究，用抗CD-31抗体对微血管进行染色，用抗胰蛋白酶抗体使肥大细胞可视化。用图像分析系统测量血管化程度和肥大细胞浸润情况。研究显示，与正常黏膜相比，OSCC病灶内和病灶周围区域的微血管密度和肥大细胞丰度显著增加。在OSCC的两个部位，微血管密度与肥大细胞浓度之间均存在显著正相关（分别为p < 0.02，p < 0.001），而在正常黏膜中未观察到类似的相关性。所得结果表明，肥大细胞在OSCC血管生成调节中起重要作用，尽管其活性的某些方面具有潜在的诊断和治疗意义，需要进一步研究。
