Danova M, Riccardi A, Mazzini G, Ucci G, Gaetani P, Silvani V, Knerich R, Butti G, Ascari E
Oncology. 1987;44(2):102-7. doi: 10.1159/000226455.
Ploidy and proliferative characteristics were estimated by flow cytometry of the nuclear DNA content of 92 human brain tumors. Samples were frozen at -20 degrees C immediately after surgery and single cell suspensions were obtained with a mechanical dissociation technique. Propidium iodide was employed for nuclear DNA staining. Human normal brain tissue was used as internal diploid reference standard. 86% of benign tumors had unimodal DNA distribution with a DNA index (DNA I = modal channel of the G0/1 peak of the studied population/modal channel of the G0/1 peak of the normal brain) usually within the diploid or near-diploid range. 14.0% had aneuploidy, with an additional cell peak having a median DNA I of 1.60. Among malignant tumors, these figures were 61.2 and 38.8% (p less than 0.001). The percentage of S phase cells was higher in malignant (median = 3.6) than in benign tumors (median = 2.0, p less than 0.01), without correlation to histological tumor subtype. Flow cytometry appears to be a useful method for evaluating differences in DNA distribution in tumors of the central nervous system.
通过对92例人脑肿瘤的核DNA含量进行流式细胞术检测，评估其倍性和增殖特性。样本在手术后立即于-20℃冷冻，采用机械解离技术获得单细胞悬液。使用碘化丙啶进行核DNA染色。以人正常脑组织作为内部二倍体参考标准。86%的良性肿瘤具有单峰DNA分布，其DNA指数（DNA I = 研究群体G0/1峰的模式通道/正常脑G0/1峰的模式通道）通常在二倍体或近二倍体范围内。14.0%为非整倍体，有一个额外的细胞峰，其中位DNA I为1.60。在恶性肿瘤中，这些数字分别为61.2%和38.8%（p < 0.001）。恶性肿瘤中S期细胞的百分比（中位数 = 3.6）高于良性肿瘤（中位数 = 2.0，p < 0.01），且与肿瘤组织学亚型无关。流式细胞术似乎是评估中枢神经系统肿瘤中DNA分布差异的一种有用方法。
