Tanvir Rabia, Sajid Imran, Hasnain Shahida
Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Punjab, Pakistan,
Biotechnol Lett. 2014 Apr;36(4):767-73. doi: 10.1007/s10529-013-1430-0. Epub 2013 Dec 29.
Endophytic actinomycetes from five Asteraceae plants were isolated and evaluated for their bioactivities. From Parthenium hysterophorus, Ageratum conyzoides, Sonchus oleraceus, Sonchus asper and Hieracium canadense, 42, 45, 90, 3, and 2 isolates, respectively, were obtained. Of the isolates, 86 (47.2 %) showed antimicrobial activity. Majority of the isolates were recovered from the roots (n = 127, 69.7 %). The dominant genus was Streptomyces (n = 96, 52.7 %), while Amycolatopsis, Pseudonocardia, Nocardia and Micromonospora were also recovered. Overall, 36 of the 86 isolates were significantly bioactivity while 18 (20.9 %) showed strong bioactivity. In total, 52.1 and 66.6 % showed potent cytotoxicity and antioxidant activities. The LC50 for 15 strains was <20 μg/ml. Compared to the ascorbate standard (EC50 0.34 μg/ml), all isolates gave impressive results with notable EC50 values of 0.65, 0.67, 0.74 and 0.79 μg/ml.
从五种菊科植物中分离出内生放线菌，并对其生物活性进行了评估。分别从银胶菊、藿香蓟、苦苣菜、刺儿菜和加拿大山柳菊中获得了42、45、90、3和2株分离物。在这些分离物中，86株（47.2%）表现出抗菌活性。大多数分离物是从根部获得的（n = 127，69.7%）。优势属为链霉菌属（n = 96，52.7%），同时也分离出了拟无枝酸菌属、假诺卡氏菌属、诺卡氏菌属和小单孢菌属。总体而言，86株分离物中有36株具有显著生物活性，18株（20.9%）表现出强生物活性。共有52.1%和66.6%的分离物表现出强效细胞毒性和抗氧化活性。15株菌株的半数致死浓度（LC50）<20μg/ml。与抗坏血酸标准品（半数有效浓度（EC50）为0.34μg/ml）相比，所有分离物都给出了令人印象深刻的结果，显著的EC50值分别为0.65、0.67、0.74和0.79μg/ml。
