Gu Xiang-Chao, Luo Xue-Gang, Wang Chong-Xi, Ma De-Yun, Wang Yan, He Ying-Ying, Li Wen, Zhou Hao, Zhang Tong-Cun
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
Biotechnol Lett. 2014 May;36(5):975-83. doi: 10.1007/s10529-013-1434-9. Epub 2013 Dec 29.
Genes coding for bile salt hydrolase of Lactobacillus plantarum CGMCC 8198, a novel probiotic strain isolated from silage, were identified, analyzed and cloned. L. plantarum strongly resisted the inhibitory effects of bile salts and also decreased serum cholesterol levels by 20% in mice with hypercholesterolemia. Using RT-PCR analysis, bsh2, bsh3 and bsh4 were upregulated by bile salts in a dose-dependent manner. All three bsh genes had high similarity with those of other Lactobacillus strains. All three recombinant BSHs had high activities for the hydrolysis of glycodeoxycholic acids and taurodeoxycholic acids.
对从青贮饲料中分离出的新型益生菌植物乳杆菌CGMCC 8198编码胆汁盐水解酶的基因进行了鉴定、分析和克隆。植物乳杆菌对胆汁盐的抑制作用具有很强的抗性，并且在高胆固醇血症小鼠中还可使血清胆固醇水平降低20%。通过RT-PCR分析，bsh2、bsh3和bsh4被胆汁盐以剂量依赖的方式上调。所有这三个bsh基因与其他乳杆菌菌株的基因具有高度相似性。所有三种重组BSH对甘氨脱氧胆酸和牛磺脱氧胆酸的水解均具有高活性。
