Zare Hakimeh, Izadi Amoli Rabeeh, Rezapour Maysam, Zaboli Fatemeh, Kaboosi Hami
Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Department of Paramedicine, Amol School of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran.
Indian J Microbiol. 2024 Jun;64(2):529-539. doi: 10.1007/s12088-023-01186-2. Epub 2024 Apr 4.
Vaginal lactobacilli protect against bacterial vaginosis and vaginal candidiasis. They may have probiotic properties and help maintain the balance and health of the vaginal ecosystem while the loss of these bacteria predisposes females to urinary and genital infections. The aim of this study was to investigate the probiotic potential of vaginal among healthy females in northern Iran. The strains were isolated from vaginal samples and were identified by sequencing of the fragment. Functional properties such as tolerance to low pH, HO production, adherence ability to Hela cells and antagonistic activity against was examined. A total of 38 vaginal lactobacilli strains from five species including (n = 13), (n = 10), (n = 6), (n = 5) and (n = 4), were identified. All of the species showed significant tolerance to low pH over 24 h ( < 0.001). The best adherence ability to Hela cells was seen in strains. Nearly 17 of the strains had higher anti- activity compared to the other strains. According to the findings, four lactobacilli strains isolated in the vaginal samples of healthy Iranian women had the best probiotic potential.
阴道乳酸杆菌可预防细菌性阴道病和阴道念珠菌病。它们可能具有益生菌特性，有助于维持阴道生态系统的平衡和健康，而这些细菌的缺失会使女性易患泌尿系统和生殖系统感染。本研究的目的是调查伊朗北部健康女性阴道内乳酸杆菌的益生菌潜力。从阴道样本中分离出菌株，并通过对片段进行测序来鉴定。检测了其对低pH的耐受性、产H2O2能力、对Hela细胞的黏附能力以及对的拮抗活性等功能特性。共鉴定出38株来自五个物种的阴道乳酸杆菌菌株，包括（n = 13）、（n = 10）、（n = 6）、（n = 5）和（n = 4）。所有物种在24小时内对低pH均表现出显著耐受性（P < 0.001）。菌株对Hela细胞的黏附能力最强。与其他菌株相比，近17株菌株具有更高的抗活性。根据研究结果，在伊朗健康女性阴道样本中分离出的四株乳酸杆菌菌株具有最佳的益生菌潜力。
