Caley Andrew, Evans Mererid, Powell Ned, Paleri Vinidh, Tomkinson Alun, Urbano Teresa Guerrero, Jay Amrita, Robinson Max, Thavaraj Selvam
Department of Clinical Oncology, Velindre Cancer Centre, Cardiff, United Kingdom.
Head Neck. 2015 Feb;37(2):202-8. doi: 10.1002/hed.23584. Epub 2014 Mar 20.
The purpose of this study was to present the clinicopathological features of a series of patients with human papillomavirus (HPV)-associated head and neck second primary tumors.
Patients with HPV-associated head and neck second primary tumors from 3 centers were identified. HPV infection was evaluated using p16 by immunohistochemistry (IHC), high-risk HPV DNA by in situ hybridization (ISH), and HPV genotyping by DNA polymerase chain reaction (PCR) enzyme immunoassay (EIA).
Eleven patients were identified: 5 with synchronous and 6 with metachronous HPV-positive second primary tumors, the latter demonstrating a mean time interval of 5 years. There were 13 second primary tumors: 11 oropharyngeal, 1 nasopharyngeal, and 1 floor of the mouth. Nine of 10 genotyped patients harbored HPV-16, and 1 patient had HPV-33 in 3 synchronous tumors.
HPV-associated second primary tumors may present as synchronous and/or metachronous lesions and can arise outside the oropharynx after prolonged intervals. Further work is necessary to identify patients at risk and to elucidate the mechanisms of HPV-associated head and neck second primary tumors.
本研究的目的是呈现一系列人乳头瘤病毒（HPV）相关头颈部第二原发肿瘤患者的临床病理特征。
确定来自3个中心的HPV相关头颈部第二原发肿瘤患者。采用免疫组织化学（IHC）检测p16评估HPV感染，采用原位杂交（ISH）检测高危HPV DNA，采用DNA聚合酶链反应（PCR）酶免疫测定（EIA）进行HPV基因分型。
共确定11例患者：5例为同步性HPV阳性第二原发肿瘤，6例为异时性HPV阳性第二原发肿瘤，后者的平均时间间隔为5年。共有13个第二原发肿瘤：11个口咽肿瘤、1个鼻咽肿瘤和1个口腔底部肿瘤。10例进行基因分型的患者中有9例携带HPV-16，1例患者在3个同步肿瘤中携带HPV-33。
HPV相关第二原发肿瘤可能表现为同步性和/或异时性病变，且在较长时间间隔后可发生于口咽以外部位。有必要进一步开展工作以确定高危患者，并阐明HPV相关头颈部第二原发肿瘤的发病机制。
