结膜相关淋巴组织的发育、改变及实时动态变化
Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue.
作者信息
Siebelmann Sebastian, Gehlsen Uta, Hüttmann Gereon, Koop Norbert, Bölke Torsten, Gebert Andreas, Stern Michael E, Niederkorn Jerry Y, Steven Philipp
机构信息
Department of Ophthalmology, University of Cologne, Cologne, Germany.
Institute of Biomedical Optics, University of Lübeck, Lübeck, Germany.
出版信息
PLoS One. 2013 Dec 20;8(12):e82355. doi: 10.1371/journal.pone.0082355. eCollection 2013.
PURPOSE
Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model.
METHODS
Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy.
RESULTS
Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 µm/min.
CONCLUSIONS
CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration.
目的
结膜相关淋巴组织（CALT）被认为在引发眼表相关免疫反应中起关键作用。本研究旨在利用一种新型小鼠模型，首次深入了解CALT在发育、细胞动力学和形态改变方面的功能。
方法
使用饲养在不同环境条件下的BALB/c小鼠，在局部抗原刺激后以及淋巴结切除和脾切除后，研究CALT的表达和形态。局部应用颗粒和细菌以研究抗原转运。使用双光子显微镜进行活体可视化。
结果
小鼠CALT的出生后发育和超微结构与人类相似。局部抗原刺激显著改变CALT表达。细菌通过淋巴上皮发生易位，而滤泡内的细胞速度约为8μm/分钟。
结论
小鼠的CALT是眼表的免疫界面，具有形态可塑性、颗粒/细菌转运和细胞迁移等动态过程。
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