Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI, USA; Department of Ophthalmology and Visual Science, Yale University, New Haven, CT, USA.
Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI, USA.
Exp Eye Res. 2024 Dec;249:110123. doi: 10.1016/j.exer.2024.110123. Epub 2024 Oct 11.
In this study we investigated the presence of resident Foxp3 regulatory T cells (Tregs) within the cornea and assessed the role of resident Tregs in corneal epithelial wound healing. Using a mouse model, we showed that in the steady state Foxp3Tregs are either in close proximity or co-localize with ABCG2 limbal stem cells. We also showed that these Tregs reside within the epithelial layer and not the corneal stroma. In addition, using a mouse model of mechanical injury, we demonstrated that depletion of Tregs from the cornea prior to corneal mechanical injury, using subconjunctival injection of anti-CD25, was associated with delayed epithelial healing. These results suggest a role for cornea resident Tregs in corneal epithelial cell function and wound healing and opens doors for further exploration of the role of Tregs in limbal stem cell function and survival.
在这项研究中，我们调查了 Foxp3 调节性 T 细胞（Tregs）在角膜中的存在情况，并评估了驻留 Tregs 在角膜上皮伤口愈合中的作用。使用小鼠模型，我们表明在稳定状态下，Foxp3Tregs 要么与 ABCG2 角膜缘干细胞接近，要么与它们共定位。我们还表明，这些 Tregs 存在于上皮层中，而不是角膜基质中。此外，使用角膜机械损伤的小鼠模型，我们通过结膜下注射抗 CD25 证明，在角膜机械损伤之前从角膜中耗尽 Tregs 与上皮愈合延迟有关。这些结果表明角膜驻留 Tregs 在角膜上皮细胞功能和伤口愈合中发挥作用，并为进一步探索 Tregs 在角膜缘干细胞功能和存活中的作用开辟了道路。
