Kamholz J, Spielman R, Gogolin K, Modi W, O'Brien S, Lazzarini R
Am J Hum Genet. 1987 Apr;40(4):365-73.
With a human myelin-basic-protein (MBP) cDNA used as a probe, the human MBP gene has been mapped to chromosome region 18q22-q23 by a combination of Southern hybridization to a panel of somatic-cell hybrid DNAs and in situ hybridization to metaphase chromosomes. Restriction-fragment-length polymorphisms (RFLPs) have also been identified with this probe in human DNA, by means of the restriction enzymes BamHI, PvuII, and PstI. In studies of informative families, the alleles of the BamHI and PvuII polymorphisms have been shown to segregate as Mendelian traits.
以人髓鞘碱性蛋白（MBP）cDNA作为探针，通过与一组体细胞杂交DNA进行Southern杂交以及与中期染色体进行原位杂交相结合的方法，已将人MBP基因定位到染色体区域18q22 - q23。利用限制性内切酶BamHI、PvuII和PstI，还通过该探针在人DNA中鉴定出了限制性片段长度多态性（RFLP）。在对信息丰富的家系进行的研究中，BamHI和PvuII多态性的等位基因已被证明以孟德尔性状进行分离。
