Bernier V, Azar H A
Am J Surg Pathol. 1987 May;11(5):387-96. doi: 10.1097/00000478-198705000-00008.
Ten cases of "undifferentiated" large-cell tumors were ultrastructurally characterized by cells with abundant filiform cytoplasmic projections without intercellular junctions. These cases were studied by means of the avidin-biotin-peroxidase complex (ABC) technique applied to formalin-fixed, paraffin-embedded sections using antibodies against high- and low-molecular weight keratins (Ker), vimentin (Vi), epithelial membrane antigen (EMA), S-100 protein, leucocyte common antigen (LCA), kappa (K) and lambda (L) light chains, Leu-M1, lysozyme (Ly), alpha-1 antitrypsin (A1AT) and alpha-1 antichymotrypsin (A1ACT). All 10 cases were negative for Ker and EMA but positive for Vi. S-100 was present only in scattered dendritic cells. LCA was identified in seven cases. In the three LCA-negative cases, two stained for Leu-M1, and one of these also showed intracytoplasmic L; one was negative for all markers but Vi. None of the tumors showed any significant staining for Ly, A1AT, or A1ACT. Our findings indicate that these tumors are nonepithelial and nonneuroectodermal, and that they are best classified as non-Hodgkin's lymphomas. The possibility that some of the filiform large-cell lymphomas may be derived from dendritic reticular cells cannot be excluded.
10例“未分化”大细胞肿瘤的超微结构特征为细胞具有丰富的丝状胞质突起且无细胞间连接。采用抗高分子量和低分子量角蛋白（Ker）、波形蛋白（Vi）、上皮膜抗原（EMA）、S-100蛋白、白细胞共同抗原（LCA）、κ（K）和λ（L）轻链、Leu-M1、溶菌酶（Ly）、α1抗胰蛋白酶（A1AT）和α1抗糜蛋白酶（A1ACT）的抗体，对福尔马林固定、石蜡包埋切片应用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）技术对这些病例进行研究。所有10例病例Ker和EMA均为阴性，但Vi为阳性。S-100仅存在于散在的树突状细胞中。7例病例检测到LCA。在3例LCA阴性的病例中，2例Leu-M1染色阳性，其中1例还显示胞质内有L；1例除Vi外所有标志物均为阴性。所有肿瘤Ly、A1AT或A1ACT均无明显染色。我们的研究结果表明，这些肿瘤既不是上皮性也不是神经外胚层性的，它们最好归类为非霍奇金淋巴瘤。不能排除一些丝状大细胞淋巴瘤可能来源于树突状网状细胞的可能性。
