Meis J M, Osborne B M, Butler J J
Am J Clin Pathol. 1986 Nov;86(5):591-9. doi: 10.1093/ajcp/86.5.591.
A comparative study of large cell lymphoma (LCL) (ten B and ten T), Hodgkin's disease (15 cases), and true histiocytic lymphoma (two cases) was undertaken, using formalin-fixed paraffin-embedded tissue sections, a panel of eight antibodies, and one lectin to determine if any particular antibody or immunologic profile could reliably distinguish between these entities. The antibodies used were against Leu-M1, alpha-1-anti-chymotrypsin (alpha-ACT), alpha-anti-trypsin (alpha-AT), lysozyme, kappa, lambda, leukocyte common antigen (LCA), and S-100 protein. The lectin used was peanut agglutinin (PNA). Although Leu-M1 staining was positive in 11 of 15 cases (73%) of Hodgkin's disease, it was also positive in 4 of 10 cases (40%) of T-cell lymphoma, 2 of 10 cases (20%) of B-cell lymphoma, and 1 of 2 cases (50%) of true histiocytic lymphoma. Peanut-agglutinin staining results were similar to Leu-M1. The only staining profile that emerged was the presence of Leu-M1, PNA-, alpha-ACT, and alpha-AT staining in Reed-Sternberg (RS) cells in 11 of 15 cases of Hodgkin's disease. Leu-M1 and its staining pattern is characteristic, but not entirely specific for RS cells, and it was not positive in at least 25% of the cases of Hodgkin's disease in formalin-fixed, paraffin-embedded tissues. The limitations of this antibody and others should be recognized.
对大细胞淋巴瘤（LCL）（10例B细胞型和10例T细胞型）、霍奇金病（15例）和真性组织细胞淋巴瘤（2例）进行了一项对比研究，采用福尔马林固定石蜡包埋的组织切片、一组8种抗体和一种凝集素，以确定是否有任何特定抗体或免疫表型能够可靠地区分这些实体。所用抗体针对Leu-M1、α1抗胰凝乳蛋白酶（α-ACT）、α抗胰蛋白酶（α-AT）、溶菌酶、κ轻链、λ轻链、白细胞共同抗原（LCA）和S-100蛋白。所用凝集素为花生凝集素（PNA）。虽然15例霍奇金病中有11例（73%）Leu-M1染色呈阳性，但10例T细胞淋巴瘤中有4例（40%）、10例B细胞淋巴瘤中有2例（20%）以及2例真性组织细胞淋巴瘤中有1例（50%）也呈阳性。花生凝集素染色结果与Leu-M1相似。唯一出现的染色模式是15例霍奇金病中有11例的里德-施特恩贝格（RS）细胞中存在Leu-M1、PNA、α-ACT和α-AT染色。Leu-M1及其染色模式具有特征性，但并非RS细胞完全特异性，在福尔马林固定石蜡包埋组织的至少25%霍奇金病病例中呈阴性。应认识到该抗体及其他抗体的局限性。
