Carvalho Y R, Loyola A M, Gomez R S, Araújo V C
Department of Oral Pathology, School of Dentistry, State University of São Paulo, Brazil.
Oral Dis. 1995 Mar;1(1):20-5. doi: 10.1111/j.1601-0825.1995.tb00152.x.
To study the nature of multinucleated and mononuclear cells from peripheral giant cell granuloma (PGCG).
Formalin-fixed, paraffin-embedded sections of 40 cases of PGCG were immunohistochemically stained for vimentin, alpha I-antichymotrypsin, CD68, S-100 protein, lysozyme, leucocyte common antigen (LCA), factor VIII-related antigen and muscle cell actin. Six cases of PGCG were also studied by transmission electron microscopy.
Vimentin, alpha I-antichymotrypsin and CD68 were expressed in both the mononuclear and multinucleated giant cells. Dendritic mononuclear cells, positive for S-100 protein, were noted in 67.5% of the lesions, whereas lysozyme and leucocyte common antigen were detected in occasional mononuclear cells. Ultrastructural examination showed mononuclear cells with signs of phagocytosis and sometimes interdigitations with similar cells. Others presented non-specific characteristics and the third type exhibited cytoplasmic processes and occasional Birbeck granules. Some multinucleated giant cells showed oval nuclei, abundant mitochondria and granular endoplasmic reticulum whereas others presented with irregular nuclei and a great number of cytoplasmic vacuoles.
Immunohistochemical and ultrastructural results suggest that PGCGs of the jaws are composed mainly of cells of the mononuclear phagocyte system and that Langerhans cells are present in two thirds of the lesions.
研究外周巨细胞肉芽肿（PGCG）中多核细胞和单核细胞的性质。
对40例PGCG的福尔马林固定、石蜡包埋切片进行免疫组织化学染色，检测波形蛋白、α1抗糜蛋白酶、CD68、S-100蛋白、溶菌酶、白细胞共同抗原（LCA）、因子VIII相关抗原和肌动蛋白。对6例PGCG还进行了透射电子显微镜检查。
波形蛋白、α1抗糜蛋白酶和CD68在单核细胞和多核巨细胞中均有表达。67.5%的病变中可见S-100蛋白阳性的树突状单核细胞，而偶尔在单核细胞中可检测到溶菌酶和白细胞共同抗原。超微结构检查显示单核细胞有吞噬迹象，有时与类似细胞有指状交叉。其他细胞呈现非特异性特征，第三种细胞表现出细胞质突起和偶尔的伯贝克颗粒。一些多核巨细胞显示椭圆形核、丰富的线粒体和颗粒内质网，而其他细胞则呈现不规则核和大量细胞质空泡。
免疫组织化学和超微结构结果表明，颌骨PGCG主要由单核吞噬细胞系统的细胞组成，三分之二的病变中有朗格汉斯细胞。
