Embrapa South Animal Husbandry & Sheep, Bagé, RS, Brazil.
Department of Pathology, Adolfo Lutz Institute, São Paulo, SP, Brazil.
Exp Parasitol. 2014 Feb;137:66-73. doi: 10.1016/j.exppara.2013.12.005. Epub 2013 Dec 28.
The experimental system of Taenia crassiceps cysticerci infection in BALB/c mice is considered to be the most representative model of cysticercosis. In our work, mice were sacrificed 7 and 30days after infection, and pouch fluid was collected to determine the number of accumulated cells and the concentrations of IFNγ, IL-2, IL-4, IL-6, IL-10 and nitric oxide. The injection of 50 nonbudding cysticerci into normal mouse dorsal air pouches induced a high level of IFNγ and nitric oxide production relative to the parasite load. The air pouch provides a convenient cavity that allows studying the cellular immunological aspects of the T. crassiceps parasite. The nonbudding cysticerci recovered from the air pouches contained cells that can reconstitute complete cysts in the peritoneal cavity of mice. In conclusion, these results demonstrate that the air pouch model is an alternative tool for the evaluation of the immune characteristics of T. crassiceps infection.
曼氏迭宫绦虫囊尾蚴感染 BALB/c 小鼠的实验系统被认为是最具代表性的囊尾蚴病模型。在我们的工作中，感染后 7 天和 30 天处死小鼠，收集囊液以确定累积细胞的数量以及 IFNγ、IL-2、IL-4、IL-6、IL-10 和一氧化氮的浓度。将 50 个非出芽囊尾蚴注入正常小鼠背部气囊中，相对于寄生虫负荷，会诱导高水平的 IFNγ 和一氧化氮产生。气囊中提供了一个方便的腔，允许研究曼氏迭宫绦虫寄生虫的细胞免疫方面。从气囊中回收的非出芽囊尾蚴含有可以在小鼠腹腔中重新构成完整囊的细胞。总之，这些结果表明气腔模型是评估曼氏迭宫绦虫感染免疫特征的一种替代工具。
