Toledo A, Cruz C, Fragoso G, Laclette J P, Merchant M T, Hernández M, Sciutto E
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F., México.
J Parasitol. 1997 Apr;83(2):189-93.
Taenia crassiceps cysticerci were disrupted through trypsinization to isolate cells which can be maintained in culture for up to 15 days. When injected intraperitoneally into susceptible BALB/cAnN mice, complete cysticerci were recovered in a number that is proportional to the quantity of injected cells. Thus, cysticerci contain cells which can reconstitute complete cysts, suggesting that individual cells play a role, independent to budding, during asexual multiplication of T. crassiceps cysticerci in the peritoneal cavity of mice. In contrast, injection of the cells into resistant C57BL/6J mice does not result in the recovery of complete cysts. These findings provide a new experimental model to identify resistance factors in the hosts, for the in vitro screening of anti-cysticerci drugs and for the genetic manipulation of cysticerci through recombinant DNA techniques.
通过胰蛋白酶消化破坏肥胖带绦虫囊尾蚴以分离细胞，这些细胞可在培养中维持长达15天。当将其腹腔注射到易感的BALB/cAnN小鼠中时，可回收数量与注射细胞量成比例的完整囊尾蚴。因此，囊尾蚴含有能够重新构建完整囊肿的细胞，这表明在小鼠腹腔内肥胖带绦虫囊尾蚴的无性繁殖过程中，单个细胞在独立于出芽的过程中发挥作用。相比之下，将这些细胞注射到抗性C57BL/6J小鼠中不会导致完整囊肿的回收。这些发现为鉴定宿主中的抗性因子、体外筛选抗囊尾蚴药物以及通过重组DNA技术对囊尾蚴进行基因操作提供了一种新的实验模型。
