İstanbul Medical Faculty Department of Orthopaedics and Traumatology, Istanbul University, Topkapı, 34390, Istanbul, Turkey,
Arch Orthop Trauma Surg. 2014 Mar;134(3):405-11. doi: 10.1007/s00402-013-1914-3. Epub 2013 Dec 31.
Platelet-rich plasma (PRP) is a natural concentrate of autologous growth factors now being widely tested in different fields of medicine for its potential in enhancing the regeneration of tissue with low healing potential. However, studies of PRP in enhancing rotator cuff repair have been contradictory, perhaps because of how PRP is administered. The purpose of this study is to evaluate the effect of PRP and compare two different application methods of PRP on rotator cuff healing.
The supraspinatus tendons of 48 mature, male Wistar-Albino rats were detached from their insertion on the humerus. The animals were divided into four groups: (1) no repair, (2) primary repair, (3) repair plus PRP injections into the tendon-bone interface, and (4) repair plus PRP absorbed from a sponge carrier to the tendon-bone interface. The tendons were evaluated biomechanically and histologically at week 8.
Cuffs repaired with PRP had significantly greater mean (SD) load-to-failure rates [11.1 (6.5) and 11.6 (3.9) N; P < 0.05] and stiffness [3.5 (2.3) and 1.6 (0.75) N; P < 0.05] than did cuffs repaired without PRP. The groups receiving PRP did not differ significantly on these variables. Histological evaluation showed no significant differences among the four groups.
The application of PRP, independent of the application method, significantly improved biomechanical properties at the rotator cuff tendon-bone interface. The type of application, injection or absorption from a sponge did not influence the effect of PRP on rotator cuff healing.
富含血小板的血浆（PRP）是一种自体生长因子的天然浓缩物，目前正在医学的不同领域中广泛测试，以研究其在增强低愈合潜能组织再生方面的潜力。然而，PRP 在增强肩袖修复方面的研究结果相互矛盾，这可能是由于 PRP 的给药方式不同。本研究旨在评估 PRP 的效果，并比较 PRP 的两种不同应用方法对肩袖愈合的影响。
将 48 只成熟的雄性 Wistar-Albino 大鼠的冈上肌腱从肱骨上的插入处分离。将动物分为四组：（1）不修复，（2）直接修复，（3）修复加 PRP 注射到肌腱-骨界面，（4）修复加 PRP 从海绵载体吸收到肌腱-骨界面。第 8 周时对肌腱进行生物力学和组织学评估。
用 PRP 修复的肩袖的失效负载率[11.1（6.5）和 11.6（3.9）N；P<0.05]和刚度[3.5（2.3）和 1.6（0.75）N；P<0.05]明显高于未用 PRP 修复的肩袖。接受 PRP 的两组在这些变量上没有显著差异。组织学评估显示四组之间无显著差异。
PRP 的应用，无论应用方式如何，均可显著改善肩袖肌腱-骨界面的生物力学性能。应用方式，即注射或从海绵吸收，并不影响 PRP 对肩袖愈合的影响。
