Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2014 Mar;105(3):523-31. doi: 10.1007/s10482-013-0104-1. Epub 2013 Dec 31.
A Gram-stain negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain, BS-R1(T), was isolated from a tidal flat at Boseong, South Korea. Strain BS-R1(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BS-R1(T) belongs to the genus Algoriphagus, clustering consistently with the type strain of Algoriphagus mannitolivorans, with which it exhibited 98.4 % sequence similarity. Sequence similarities between strain BS-R1(T) and the type strains of the other Algoriphagus species were between 92.7 and 97.0 %. Strain BS-R1(T) was found to contain MK-7 as the predominant menaquinone and iso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The major polar lipids of strain BS-R1(T) were identified as phosphatidylcholine, phosphatidylethanolamine and one unidentified lipid. The DNA G+C content was determined to be 42.3 mol% and its mean DNA-DNA relatedness values with A. mannitolivorans KACC 11349(T) was 17 ± 5 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties demonstrated that strain BS-R1(T) is distinguishable from the other Algoriphagus species as well as A. mannitolivorans. On the basis of the data presented, strain BS-R1(T) is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus boseongensis sp. nov. is proposed. The type strain is BS-R1(T) (=KCTC 32580(T) = CECT 8446(T)).
一株革兰氏阴性、需氧、不运动、不产芽孢的杆状细菌菌株 BS-R1(T) 从韩国保宁市的潮滩中分离得到。BS-R1(T) 菌株的最佳生长条件为 30°C、pH7.0-8.0 和 2.0%（w/v）NaCl。基于 16S rRNA 基因序列的系统发育分析表明，BS-R1(T) 菌株属于 Algoriphagus 属，与 Algoriphagus mannitolivorans 模式菌株聚为一簇，序列相似性为 98.4%。BS-R1(T) 菌株与其他 Algoriphagus 种的模式菌株的序列相似性在 92.7%至 97.0%之间。BS-R1(T) 菌株被发现含有 MK-7 作为主要的menaquinone 和 iso-C15:0、iso-C17:0 3-OH 和总和特征 3（C16:1 ω7c 和/或 C16:1 ω6c）作为主要的脂肪酸。BS-R1(T) 菌株的主要极性脂为磷脂酰胆碱、磷脂酰乙醇胺和一种未鉴定的脂。菌株 BS-R1(T) 的 DNA G+C 含量为 42.3 mol%，与 A. mannitolivorans KACC 11349(T) 的平均 DNA-DNA 同源性值为 17±5%。系统发育和遗传独特性以及表型差异表明，BS-R1(T) 菌株与其他 Algoriphagus 种以及 A. mannitolivorans 都不同。基于所提出的数据，BS-R1(T) 菌株被认为代表了 Algoriphagus 属的一个新种，命名为 Algoriphagus boseongensis sp. nov.。模式菌株为 BS-R1(T)=KCTC 32580(T)=CECT 8446(T)。
