Park Sooyeon, Kim Sona, Jung Yong-Taek, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
Int J Syst Evol Microbiol. 2016 Jan;66(1):118-124. doi: 10.1099/ijsem.0.000686. Epub 2015 Oct 14.
A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated HJM-2T, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain HJM-2T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain HJM-2T belonged to the genus Algoriphagus, clustering coherently with the type strain of A. taiwanensis. Strain HJM-2T exhibited 16S rRNA gene sequence similarities of 98.6 and 97.2 % to A. taiwanensis CC-RR-82T and A. boseongensis BS-R1T, respectively, and 92.7-96.7 % to the type strains of the other species of the genus Algoriphagus. Strain HJM-2T contained MK-7 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain HJM-2T were phosphatidylcholine, phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain HJM-2T was 45 mol% and mean DNA-DNA relatedness values with the type strains of A. taiwanensis and A. boseongensis were 10-19 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain HJM-2T is separated from recognized species of the genus Algoriphagus. On the basis of the data presented, strain HJM-2T represents a novel species of the genus Algoriphagus, for which the name Algoriphagus confluentis sp. nov. is proposed. The type strain is HJM-2T ( = KCTC 42704T = NBRC 111222T).
从韩国华津浦海洋与淡水湖交汇处分离出一株革兰氏阴性、无鞭毛、不滑动、需氧的杆状细菌，命名为HJM-2T，并采用多相分类法对其进行分类学研究。菌株HJM-2T在30 °C、pH 7.0 - 8.0以及1.0 - 2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株HJM-2T属于嗜冷杆菌属，与台湾嗜冷杆菌模式菌株紧密聚类。菌株HJM-2T与台湾嗜冷杆菌CC-RR-82T和保宁嗜冷杆菌BS-R1T的16S rRNA基因序列相似性分别为98.6%和97.2%，与嗜冷杆菌属其他种模式菌株的相似性为92.7 - 96.7%。菌株HJM-2T以MK-7作为主要甲基萘醌，以异-C15 : 0、特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）以及异-C17 : 0 3-OH作为主要脂肪酸。在菌株HJM-2T中检测到的主要极性脂为磷脂酰胆碱、磷脂酰乙醇胺和一种未鉴定的脂质。菌株HJM-2T的DNA G+C含量为45 mol%，与台湾嗜冷杆菌和保宁嗜冷杆菌模式菌株的平均DNA-DNA相关性值为10 - 19 %。不同的表型特征，以及其系统发育和遗传独特性，表明菌株HJM-2T与嗜冷杆菌属已确认的种不同。根据所提供的数据，菌株HJM-2T代表嗜冷杆菌属的一个新种，为此提出新名称融合嗜冷杆菌（Algoriphagus confluentis sp. nov.）。模式菌株为HJM-2T（ = KCTC 42704T = NBRC 111222T）。
