Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):200-204. doi: 10.1099/ijs.0.012682-0. Epub 2009 Jul 31.
A Gram-negative, non-motile, non-spore-forming bacterial strain, S1-3(T), was isolated from a tidal flat sediment on the west coast of Korea and its taxonomic position was investigated. Strain S1-3(T) grew optimally at 30 degrees C and in the presence of 2 % (w/v) NaCl. Strain S1-3(T) contained MK-7 as the predominant menaquinone and C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH and iso-C(15 : 0) as the major fatty acids. The DNA G+C content was 41.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain S1-3(T) fell within the clade comprising Algoriphagus species, clustering with Algoriphagus halophilus IMSNU 14013(T), with which it exhibited 99.6 % 16S rRNA gene sequence similarity. The 16S rRNA gene sequence similarity between strain S1-3(T) and the type strains of other Algoriphagus species was 94.0-97.1 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain S1-3(T) demonstrated that this strain is distinguishable from the other Algoriphagus species as well as A. halophilus. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain S1-3(T) is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus lutimaris sp. nov. is proposed. The type strain is S1-3(T) (=KCTC 22630(T) =CCUG 57608(T)).
一株革兰氏阴性、非运动性、非孢子形成的细菌菌株 S1-3(T) 从韩国西海岸的潮滩沉积物中分离出来，并对其分类地位进行了研究。S1-3(T) 菌株在 30°C 和 2%（w/v）NaCl 存在下生长最佳。S1-3(T) 菌株含有 MK-7 作为主要的menaquinone，C(16:1)ω7c 和/或 iso-C(15:0) 2-OH 和 iso-C(15:0) 作为主要的脂肪酸。DNA G+C 含量为 41.4 mol%。基于 16S rRNA 基因序列的系统发育分析表明，S1-3(T) 菌株属于 Algoriphagus 种的分支，与 Algoriphagus halophilus IMSNU 14013(T)聚在一起，其 16S rRNA 基因序列相似性为 99.6%。S1-3(T) 菌株与其他 Algoriphagus 种的模式菌株的 16S rRNA 基因序列相似性为 94.0-97.1%。S1-3(T) 菌株的表型特征、化学分类特征、系统发育和遗传特征表明，该菌株与其他 Algoriphagus 种以及 A. halophilus 明显不同。基于表型、化学分类、系统发育和遗传数据，S1-3(T) 菌株被认为代表了 Algoriphagus 属的一个新种，建议将其命名为 Algoriphagus lutimaris sp. nov.。模式菌株为 S1-3(T)=KCTC 22630(T)=CCUG 57608(T)。
