Naval General Hospital, PLA, Beijing, China.
Eur Rev Med Pharmacol Sci. 2013 Dec;17(24):3376-80.
This study assessed the relationship between the p53-dependent apoptosis and recurrent spontaneous abortion (RSA).
Thirty women with recurring miscarriages were enrolled as experimental group, and 30 women with normal reproduction served as control group. Immunohistochemistry was used to evaluate expression of p53 in villous tissue specimens. Further, expressions of CDKN1A and Bax mRNAs were evaluated by qPCR. TUNEL assay was utilized to document cell apoptosis.
Expression of p53 was significantly increased in chorionic villi of patients in experimental group (p < 0.05 vs. control group). Further, CDKN1A and Bax mRNA levels were elevated in experimental group (p < 0.05 vs. control group), and the cell apoptosis index was increased as well.
The p53-CDKN1A and p53-Bax signaling pathways appear to be activated in RSA. Thus, the apoptosis pathways controlled by p.
本研究旨在评估 p53 依赖性细胞凋亡与复发性自然流产（RSA）之间的关系。
招募 30 名反复流产的女性作为实验组，30 名正常妊娠的女性作为对照组。采用免疫组织化学法检测绒毛组织中 p53 的表达情况。进一步采用 qPCR 法检测 CDKN1A 和 BaxmRNA 的表达情况。TUNEL 法检测细胞凋亡情况。
实验组绒毛中 p53 的表达明显升高（p<0.05 与对照组比较）。此外，实验组 CDKN1A 和 BaxmRNA 水平升高（p<0.05 与对照组比较），细胞凋亡指数增加。
p53-CDKN1A 和 p53-Bax 信号通路在 RSA 中似乎被激活。因此，p 调控的凋亡途径。
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