Li Shuhong, Li Ning, Zhu Peng, Wang Yanwei, Tian Yongjie, Wang Xietong
Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan, China.
Department of Obstetrics and Gynecology, Yantai Yuhuangding Hospital, Yantai, China.
J Obstet Gynaecol Res. 2015 Jun;41(6):904-11. doi: 10.1111/jog.12647. Epub 2014 Dec 16.
We aimed to study the relation between Wnt/β-catenin signaling pathway and recurrent spontaneous abortion through investigating the expression of β-catenin and Dickkof-1 in first-trimester villi and decidua of recurrent spontaneous abortion patients.
Villous and decidual tissues were collected from 40 women (20 patients with recurrent spontaneous abortion and 20 patients with normal, early pregnancy). Western blots were used to measure the protein levels of β-catenin in villi and decidua, and the localization of β-catenin was investigated by immunohistochemistry. Quantitative real-time reverse transcription polymerase chain reaction was used to quantify the mRNA levels of β-catenin and Dickkof-1 in villi and decidua, respectively.
Our results indicated that β-catenin was expressed predominantly in plasma membranes of the villous cytotrophoblasts and glandular epithelium. What's more, its expression significantly decreased at both mRNA and protein levels, whereas the mRNA levels of Dickkof-1 significantly increased in villi and decidua of the recurrent spontaneous abortion group compared with the normal control group.
We therefore speculated that the downregulated Wnt/β-catenin signaling pathway might be associated with the process of the pathogenesis of recurrent spontaneous abortion.
通过研究复发性自然流产患者孕早期绒毛和蜕膜中β-连环蛋白及Dickkof-1的表达，探讨Wnt/β-连环蛋白信号通路与复发性自然流产的关系。
收集40例女性（20例复发性自然流产患者和20例正常早孕患者）的绒毛和蜕膜组织。采用蛋白质免疫印迹法检测绒毛和蜕膜中β-连环蛋白的蛋白水平，免疫组织化学法检测β-连环蛋白的定位。采用实时定量逆转录聚合酶链反应分别定量检测绒毛和蜕膜中β-连环蛋白及Dickkof-1的mRNA水平。
结果表明，β-连环蛋白主要表达于绒毛细胞滋养层细胞膜和腺上皮细胞膜。此外，与正常对照组相比，复发性自然流产组绒毛和蜕膜中β-连环蛋白的mRNA和蛋白水平均显著降低，而Dickkof-1的mRNA水平显著升高。
因此推测，Wnt/β-连环蛋白信号通路下调可能与复发性自然流产的发病机制有关。
