克罗恩病患者血清胰岛素样生长因子结合蛋白 5 浓度。

Serum concentrations of insulin-like growth factor-binding protein 5 in Crohn's disease.

机构信息

Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.

出版信息

World J Gastroenterol. 2013 Dec 21;19(47):9049-56. doi: 10.3748/wjg.v19.i47.9049.

DOI:10.3748/wjg.v19.i47.9049

PMID:24379630

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3870558/

Abstract

AIM

To investigate serum insulin-like growth factor-binding protein 5 (IGFBP-5) levels and intestinal IGFBP-5 expression in patients with Crohn's disease (CD).

METHODS

We analyzed the serum concentrations and intestinal expression of IGFBP-5 in 42 patients with CD, of whom 26 had endoscopically or radiologically proven stricture formation. Nine of the 42 patients had active disease, with a Crohn's disease activity index > 150. Serum IGFBP-5 levels were analyzed in 20 healthy controls matched by sex and age to the CD patients. Serum IGFBP-5 was measured using an enzyme-linked immunosorbent assay. Intestinal tissue was obtained from patients through endoscopic biopsies. IGFBP-5 expression was detected using immunohistochemistry and was scored semiquantitatively.

RESULTS

The median serum IGFBP-5 concentrations of CD patients were significantly lower compared with healthy controls [median 7.2 (IQR: 5.5-11.3) ng/mL vs 11.3 (8.0-44.6) ng/mL, P < 0.001]. There was no significant difference between median serum IGFBP-5 levels in CD patients with or without stricture formation [6.9 (5.5-11.3) ng/mL vs 7.8 (5.3-10.1) ng/mL, P = 0.815]. The serum IGFBP-5 levels were not significantly different between patients with active disease and inactive disease [7.2 (6.5-7.6) ng/mL vs 7.2 (5.5-11.3) ng/mL, P = 0.890]. However, a significant correlation was observed between serum IGFBP-5 levels and platelet count (PLT) (r = 0.319, P = 0.0395). No significant correlation was found between tissue IGFBP-5 immunohistochemical staining intensity scores and serum IGFBP-5 levels. No significant difference was found when comparing the serum IGFBP-5 levels among the patients with different tissue IGFBP-5 staining scores (absent/very weak, weak, moderate or strong). There was a significant correlation between tissue IGFBP-5 staining scores and white blood cell count (r = 0.391, P = 0.01) and PLT (r = 0.356, P = 0.021).

CONCLUSION

Our results indicate that serum IGFBP-5 concentrations were lower in CD patients compared to healthy controls regardless of disease activity or the presence of stricture formation.

摘要

目的

研究克罗恩病（CD）患者血清胰岛素样生长因子结合蛋白 5（IGFBP-5）水平和肠道 IGFBP-5 表达。

方法

我们分析了 42 例 CD 患者的血清浓度和肠道 IGFBP-5 表达，其中 26 例经内镜或影像学证实存在狭窄形成。42 例患者中有 9 例为活动期疾病，克罗恩病活动指数>150。通过与 CD 患者相匹配的性别和年龄，选择 20 名健康对照者分析血清 IGFBP-5 水平。采用酶联免疫吸附试验测定血清 IGFBP-5 水平。通过免疫组织化学检测肠道组织中 IGFBP-5 的表达，并进行半定量评分。

结果

与健康对照组相比，CD 患者的中位血清 IGFBP-5 浓度明显较低[中位数 7.2（IQR：5.5-11.3）ng/ml 比 11.3（8.0-44.6）ng/ml，P<0.001]。有或无狭窄形成的 CD 患者的中位血清 IGFBP-5 水平无显著差异[6.9（5.5-11.3）ng/ml 比 7.8（5.3-10.1）ng/ml，P=0.815]。活动期和非活动期疾病患者的血清 IGFBP-5 水平无显著差异[7.2（6.5-7.6）ng/ml 比 7.2（5.5-11.3）ng/ml，P=0.890]。然而，血清 IGFBP-5 水平与血小板计数（PLT）呈显著正相关（r=0.319，P=0.0395）。组织 IGFBP-5 免疫组化染色强度评分与血清 IGFBP-5 水平之间无显著相关性。不同组织 IGFBP-5 染色评分（无/弱、弱、中或强）的患者之间血清 IGFBP-5 水平无显著差异。组织 IGFBP-5 染色评分与白细胞计数（r=0.391，P=0.01）和 PLT（r=0.356，P=0.021）呈显著正相关。