Singh Hp, Shetty Dc, Kumar A, Chavan R, Shori Dd, Mali J
Department of Oral and Maxillofacial Pathology and Microbiology, Dasmesh Institute of Research and Dental Sciences, Faridkot, Punjab, India.
Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Ghaziabad, Uttar Pradesh, India.
Ann Med Health Sci Res. 2013 Oct;3(4):523-8. doi: 10.4103/2141-9248.122072.
Although, odontogenickeratocyst (OKC) and dentigerous cyst (DC) are considered asdevelopmental cysts inflammation has been seen in its connective tissue wall. Inflammation is seen to alter the epithelial lining of both OKC and DC butwhether it plays in altering the behavior of these cysts is not fully understood.
The present study is conducted with the help of molecular marker proliferating cellular nuclear antigen (PCNA) to assess the proliferative activity of OKC and DC and to further evaluate and correlate the effect of inflammation on the proliferative activity and hence on biological behavior of these cysts.
Immunohistochemicalstaining was performed using anti-PCNA antibody in 10 cases each of classical OKC, inflamed OKC, classical DC, and inflamed DC. The resulting data was tabulated on Microsoft excel and subjected to statistical analysis using two-way analysis of variance test, t test and post-hoc test followed by Bonferroni test with the application of statistical package for social sciences (SPSS) (SPSS, Chicago, IL, USA) and Epi-Info 6.04 d, Atlanta, Georgia (USA).
Total mean PCNA expression is statistically higher in inflamed OKC than classical OKC and inflamed DC also showed significantly higher PCNA expression than classical DC (P < 0.001). Correlation between inflammation and PCNA expression was not statistically significant (P ≥ 0.05).
Inflammation is responsible for change in behavior of neoplastic epithelium of OKC, whereas in dentigerous it is responsible for changes in epithelial lining and hence should be treated meticulously.
尽管牙源性角化囊肿（OKC）和含牙囊肿（DC）被认为是发育性囊肿，但其结缔组织壁中可见炎症。炎症可改变OKC和DC的上皮衬里，但炎症是否影响这些囊肿的行为尚未完全明确。
本研究借助分子标志物增殖细胞核抗原（PCNA）评估OKC和DC的增殖活性，并进一步评估和关联炎症对增殖活性的影响，进而对这些囊肿生物学行为的影响。
采用抗PCNA抗体对10例经典OKC、炎性OKC、经典DC和炎性DC分别进行免疫组织化学染色。所得数据录入Microsoft excel表格，并使用社会科学统计软件包（SPSS）（美国伊利诺伊州芝加哥市SPSS公司）和美国佐治亚州亚特兰大市的Epi-Info 6.04 d进行双向方差分析、t检验和事后检验，随后进行Bonferroni检验。
炎性OKC的PCNA总平均表达在统计学上高于经典OKC，炎性DC的PCNA表达也显著高于经典DC（P < 0.001）。炎症与PCNA表达之间的相关性无统计学意义（P≥0.0�5）。
炎症是OKC肿瘤上皮行为改变的原因，而在含牙囊肿中，炎症是上皮衬里改变的原因，因此应谨慎处理。
