利用流感病毒核蛋白的合成肽来确定由I类限制性细胞毒性T淋巴细胞识别的表位。
Use of synthetic peptides of influenza nucleoprotein to define epitopes recognized by class I-restricted cytotoxic T lymphocytes.
作者信息
Bastin J, Rothbard J, Davey J, Jones I, Townsend A
出版信息
J Exp Med. 1987 Jun 1;165(6):1508-23. doi: 10.1084/jem.165.6.1508.
Abstract
The conserved epitopes of influenza nucleoprotein (NP) recognized by class I MHC-restricted CTL from CBA (H-2k) and C57BL/10 (H-2b) mice have been defined in vitro with synthetic peptides 50-63 and 365-379, respectively. Two Db-restricted clones were described that recognize different epitopes on peptide 365-379. Finally, the recognition of complete NP was shown to be approximately 200-fold less efficient than peptide in the cytotoxicity assay. These phenomena are closely related to results with class II-restricted T cells and they strengthen the hypothesis that influenza proteins are degraded in the infected cell before recognition by class I-restricted CTL.
摘要
来自CBA（H-2k）和C57BL/10（H-2b）小鼠的I类MHC限制性CTL识别的流感核蛋白（NP）保守表位，已分别在体外利用合成肽50-63和365-379得以确定。描述了两个Db限制性克隆，它们识别肽365-379上不同的表位。最后，在细胞毒性试验中，对完整NP的识别效率显示比肽低约200倍。这些现象与II类限制性T细胞的结果密切相关，并且强化了流感蛋白在被I类限制性CTL识别之前在感染细胞中被降解的假说。
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