Yewdell J W, Frank E, Gerhard W
J Immunol. 1981 May;126(5):1814-9.
A panel of hybridoma antibodies was used to study the expression of the 2 major internal influenza A virus proteins, matrix protein (M), and nucleoprotein (NP) on the surface of influenza A-infected P815 cells. Using a sensitive radioimmunoassay (RIA) performed with live infected P815 cells only minute amounts of M were detectable, whereas M was not detected in 3 other cell-surface assays. In contrast, NP was detected in 10-fold greater amounts than M and 10- to 50-fold lower amounts than the viral glycoproteins by RIA. Furthermore, the presence of NP could be demonstrated by immunofluorescence, microcytotoxicity, and agglutination assays performed on live infected cells.
一组杂交瘤抗体被用于研究甲型流感病毒的两种主要内部蛋白，即基质蛋白（M）和核蛋白（NP）在甲型流感病毒感染的P815细胞表面的表达情况。仅使用活的感染P815细胞进行灵敏的放射免疫测定（RIA），只能检测到微量的M，而在其他三种细胞表面测定中未检测到M。相比之下，通过RIA检测到的NP量比M多10倍，比病毒糖蛋白少10至50倍。此外，通过对活的感染细胞进行免疫荧光、微量细胞毒性和凝集试验，可以证明NP的存在。
