George A, Nair R, Rath S, Ghosh S N, Kamat R S
J Med Microbiol. 1987 May;23(3):239-46. doi: 10.1099/00222615-23-3-239.
The effects of diverse factors, such as route of immunisation, composition of immunogen and administration of interferon inducer, on the expression of cell-mediated immune responses against Salmonella enteritidis were investigated in BALB/c and Swiss white mice. Immunisation with live cells of S. enteritidis by the intraperitoneal route (ip) generated both delayed type hypersensitivity (DTH) and protective cell-mediated immunity (CMI). However, the two responses showed diametrically opposite time kinetics. The decline and disappearance by 9 weeks after ip immunisation of DTH and the rise of protective immunity in the same period suggested the possibility that the two responses were mediated by different subsets of T cells. Immunisation by the intradermal (id) route with a sonicate of S. enteritidis generated only DTH; id immunisation also suppressed the development of the protective response following ip immunisation with live S. enteritidis. Both responses were not seen when T cells were eliminated with anti-T cell serum. Oral immunisation with live cells of S. enteritidis induced excellent CMI expressing both DTH and protective responses. On the other hand, oral immunisation with the sonicate of S. enteritidis not only did not induce CMI, but also prevented the development of the DTH and protective response to ip immunisation with live S. enteritidis. Induction of interferon by the administration of poly I: poly C for four consecutive days after id immunisation with killed S. enteritidis suppressed the generation of DTH.
在BALB/c和瑞士小白鼠中，研究了多种因素，如免疫途径、免疫原组成和干扰素诱导剂的给予，对针对肠炎沙门氏菌的细胞介导免疫反应表达的影响。通过腹腔内途径（ip）用肠炎沙门氏菌活细胞免疫可产生迟发型超敏反应（DTH）和保护性细胞介导免疫（CMI）。然而，这两种反应呈现出完全相反的时间动力学。腹腔内免疫9周后DTH的下降和消失以及同期保护性免疫的增强表明，这两种反应可能由不同的T细胞亚群介导。用肠炎沙门氏菌超声裂解物通过皮内（id）途径免疫仅产生DTH；皮内免疫也抑制了用肠炎沙门氏菌活细胞腹腔内免疫后保护性反应的发展。用抗T细胞血清清除T细胞时，两种反应均未出现。用肠炎沙门氏菌活细胞口服免疫诱导出了良好的CMI，表现为DTH和保护性反应。另一方面，用肠炎沙门氏菌超声裂解物口服免疫不仅未诱导CMI，而且还阻止了对用肠炎沙门氏菌活细胞腹腔内免疫的DTH和保护性反应的发展。在用灭活的肠炎沙门氏菌皮内免疫后连续四天给予聚肌苷酸：聚胞苷酸诱导干扰素，抑制了DTH的产生。
