Yokoyama H, Ikedo M, Kohbata S, Ezaki T, Yabuuchi E
Microbiol Immunol. 1987;31(1):1-11. doi: 10.1111/j.1348-0421.1987.tb03063.x.
Scanning electron micrograph of HeLa S3 monolayered cells, inoculated with viable bacteria of a Salmonella typhi strain GIFU 10007, revealed that the extended microvilli tangled the bacteria within 10 min after inoculation. The micrographs of HeLa cells, at 1 hr after inoculation, indicate the following: shortening of bacterium-attached microvilli, subsiding of tangled bacteria into microvilli bush, and then attachment of bacterial soma to cell surface making the cell membrane depressed. The transmission electron micrographs, at 1 hr after inoculation, demonstrated the findings of interaction between HeLa cell and S. typhi 10007, similar to those observed on scanning electron micrographs. Hair-like fine structures from the soma of challenge organisms were also observed. They were in contact with HeLa cell microvilli and cell membrane. The bacteria were first partially and then totally surrounded by the HeLa cell plasma membrane. One, two, or several bacteria with intact outer membrane were enclosed in intracytoplasmic membrane-bound vacuoles. Fragmented vacuolar membrane was still visible around the intracellularly accumulated bacteria at 24 hr after inoculation. The viable cells of S. typhi 10007 are regarded as internalizing into HeLa cells by a process of endocytosis and to multiply within the membrane-bound vacuoles.
接种伤寒沙门氏菌GIFU 10007活细菌的HeLa S3单层细胞的扫描电子显微镜图像显示，接种后10分钟内，伸出的微绒毛将细菌缠住。接种后1小时的HeLa细胞显微照片显示如下情况：附着细菌的微绒毛缩短，缠结的细菌沉入微绒毛丛中，然后细菌菌体附着于细胞表面，使细胞膜凹陷。接种后1小时的透射电子显微镜图像显示了HeLa细胞与伤寒沙门氏菌10007之间相互作用的结果，与扫描电子显微镜图像中观察到的结果相似。还观察到攻击生物体菌体的毛发状精细结构。它们与HeLa细胞微绒毛和细胞膜接触。细菌首先被HeLa细胞质膜部分包围，然后被完全包围。一个、两个或几个具有完整外膜的细菌被包裹在胞质内膜结合的液泡中。接种后24小时，在细胞内聚集的细菌周围仍可见破碎的液泡膜。伤寒沙门氏菌10007的活细胞被认为是通过内吞作用内化到HeLa细胞中，并在膜结合的液泡内繁殖。
