Kohbata S, Yokoyama H, Yabuuchi E
Microbiol Immunol. 1986;30(12):1225-37. doi: 10.1111/j.1348-0421.1986.tb03055.x.
An electron microscopic study revealed that, within 30 min after inoculation into the ligated ileal loop of anesthetized mice, cells of Salmonella typhi GIFU 10007 adhered to the M cell surface of Peyer's patch lymphoid follicle epithelium, and induced almost complete destruction of M cells. The M cell cytoplasms were pinched off and extruded from the epithelial lining into the luminal space together with the lymphoid cells primarily enfolded into the corresponding M cells. When two or more M cells were destroyed, a large defect in the epithelial lining was apparent, and a number of bacteria appeared near the basal lamina of the epithelial lining. These findings suggest, as far as anesthetized murine ileal loops and strain 10007 are concerned, that ileal M cells are the target cell at an early stage of S. typhi infection and the infection may further progress to deeper tissues and to the general circulation.
电子显微镜研究显示，在接种到麻醉小鼠的结扎回肠袢后30分钟内，伤寒沙门氏菌GIFU 10007的细胞粘附于派尔集合淋巴结滤泡上皮的M细胞表面，并几乎导致M细胞完全破坏。M细胞的细胞质被挤掉，并与主要包裹在相应M细胞内的淋巴细胞一起从上皮内衬挤出到管腔空间。当两个或更多M细胞被破坏时，上皮内衬出现大的缺损，并且在上皮内衬的基底层附近出现许多细菌。就麻醉的小鼠回肠袢和10007菌株而言，这些发现表明，回肠M细胞是伤寒沙门氏菌感染早期的靶细胞，并且感染可能进一步发展到更深的组织和全身循环。
