Abergel R P, Meeker C A, Oikarinen H, Oikarinen A I, Uitto J
Arch Dermatol. 1985 May;121(5):632-5.
Recent observations have suggested that retinoids might affect the metabolism of the extracellular matrix of connective tissues. In this study, we examined the effects of tretinoin (all-trans-retinoic acid) and isotretinoin (13-cis-retinoic acid) on the production of procollagen in keloid fibroblast cultures that were characterized by enhanced procollagen synthesis in vitro. The activities of three enzymes relevant to connective tissue metabolism, prolyl hydroxylase, collagenase, and an elastaselike neutral protease were also determined. The results demonstrated that collagen production was markedly reduced in cultures treated with either one of the retinoids. The activity of prolyl hydroxylase, a key enzyme in the intracellular biosynthesis of collagen, was not affected, while the production of collagenase was markedly reduced by the retinoids. In contrast, the activity of an elastaselike neutral protease in the cell culture medium was markedly enhanced by both retinoids. The results, therefore, indicate a differential modulation of connective tissue metabolism by retinoids in keloid cell cultures.
最近的观察结果表明，类视黄醇可能会影响结缔组织细胞外基质的代谢。在本研究中，我们检测了维甲酸（全反式维甲酸）和异维甲酸（13 - 顺式维甲酸）对瘢痕疙瘩成纤维细胞培养物中前胶原产生的影响，这些培养物的特征是在体外前胶原合成增强。还测定了与结缔组织代谢相关的三种酶，即脯氨酰羟化酶、胶原酶和一种类弹性蛋白酶中性蛋白酶的活性。结果表明，用任何一种类视黄醇处理的培养物中胶原产生均显著减少。脯氨酰羟化酶是细胞内胶原生物合成中的关键酶，其活性未受影响，而类视黄醇显著降低了胶原酶的产生。相反，两种类视黄醇均显著增强了细胞培养基中类弹性蛋白酶中性蛋白酶的活性。因此，结果表明类视黄醇对瘢痕疙瘩细胞培养物中结缔组织代谢具有差异性调节作用。
