植物中基因编码 pH 传感器的发展和特性。
Development and properties of genetically encoded pH sensors in plants.
机构信息
Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2 Montpellier, France.
Laboratory of Tropical and Mediterranean Symbioses (UMR113, Université Montpellier 2, Institut de Recherche pour le Développement, Cirad Montpellier SupAgro, Institut National de la Recherche Agronomique), Université Montpellier 2 Montpellier, France.
出版信息
Front Plant Sci. 2013 Dec 18;4:523. doi: 10.3389/fpls.2013.00523. eCollection 2013.
Fluorescent proteins (FPs) have given access to a large choice of live imaging techniques and have thereby profoundly modified our view of plant cells. Together with technological improvement of imaging, they have opened the possibility to monitor physico-chemical changes within cells. For this purpose, a new generation of FPs has been engineered. For instance, pHluorin, a point mutated version of green fluorescent protein, allows to get local pH estimates. In this paper, we will describe how genetically encoded sensors can be used to measure pH in the microenvironment of living tissues and subsequently discuss the role of pH in (i) exocytosis, (ii) ion uptake by plant roots, (iii) cell growth, and (iv) protein trafficking.
荧光蛋白（FPs）为多种活体成像技术提供了可能，从而极大地改变了我们对植物细胞的认识。随着成像技术的不断改进，它们为监测细胞内的物理化学变化提供了可能。为此，人们已经设计出了新一代的荧光蛋白。例如，pHluroin 是绿色荧光蛋白的一个点突变版本，它可以用来估计局部 pH 值。在本文中，我们将描述如何利用遗传编码的传感器来测量活组织微环境中的 pH 值，并随后讨论 pH 值在以下方面的作用：（i）胞吐作用；（ii）植物根系对离子的摄取；（iii）细胞生长；（iv）蛋白质运输。
Zotero 插件