Pennings A, Speth P, Wessels H, Haanen C
Cytometry. 1987 May;8(3):335-8. doi: 10.1002/cyto.990080315.
Acridine orange (AO) staining enables flow cytometric measurement of cellular DNA and RNA. With the conventional procedure, red and green fluorescence intensities alter considerably in time, affecting the reliability and reproducibility of the results. A standardized simple technique of AO staining is described that keeps the time between addition of detergent and AO and the subsequent measurement constant. Measurements by means of this on-line method appear to be much more reproducible in comparison to those obtained using the conventional procedure.
吖啶橙（AO）染色可实现细胞DNA和RNA的流式细胞术测量。按照传统方法，红色和绿色荧光强度会随时间发生显著变化，从而影响结果的可靠性和可重复性。本文描述了一种标准化的简单AO染色技术，该技术可使加入去污剂和AO之间的时间以及后续测量保持恒定。与使用传统方法获得的测量结果相比，通过这种在线方法进行的测量似乎具有更高的可重复性。
