Melamed M R, Darzynkiewicz Z, Traganos F, Sharpless T K
Cancer Res. 1977 Apr;37(4):1227-31.
Two human bladder cell lines, T-24 and HCV-29, are studied with flow cytofluorometry and acridine orange staining to determine relative DNA and RNA content per cell and to measure resistance to thermal denaturation of DNA in situ. The RNA/DNA ratio for HCV-29 is over twofold higher than that for T-24, a difference that is consistent with the differences in cytological morphology and staining characteristics of these two cell lines and is sufficient to distinguish them completely, although measurements of DNA or RNA alone may not. In addition, the two cell lines show differences in DNA "melting" curves that indicate structural or conformational differences in nuclear chromatin. It is evident that the features are related to nuclear and cellular morphology, and they may be of value as additional parameters for characterizing tissue culture cell lines.
利用流式细胞荧光测定法和吖啶橙染色法对两种人膀胱细胞系T-24和HCV-29进行研究，以确定每个细胞的相对DNA和RNA含量，并原位测量DNA对热变性的抗性。HCV-29的RNA/DNA比值比T-24高出两倍多，这一差异与这两种细胞系在细胞学形态和染色特性上的差异一致，足以将它们完全区分开来，尽管单独测量DNA或RNA可能无法做到。此外，这两种细胞系在DNA“熔解”曲线上存在差异，表明核染色质存在结构或构象差异。很明显，这些特征与细胞核和细胞形态有关，它们可能作为表征组织培养细胞系的附加参数具有价值。
