Guo Y L
Institute of Urology, Beijing Medical University.
Zhonghua Wai Ke Za Zhi. 1992 Nov;29(12):777-80, 799-800.
A panel of specific mouse monoclonal antibodies, BIU-H4, BIU-H6, BIU-H9, was obtained by immunization with BIU-87 cells (a transitional cell carcinoma cell line of the bladder), which was blocked by the serum of mouse anti-human lymphocyte. Detailed descriptions were given concerning their reactivity patterns by ABC-ELISA and IIF assays against various cells. They showed positive reactions with transitional cell carcinoma of the bladder and negative with normal bladder. BIU-H6 did not react with any of the other normal or malignant tissues. The subclass of immunoglobulin for BIU-H4,6 was IgG1, for BIU-H9 is IgG2 alpha. The molecular weight of the antigen recognized by BIU-H6,9 is 102 KD.
通过用BIU - 87细胞（膀胱移行细胞癌细胞系）免疫获得了一组特异性小鼠单克隆抗体，即BIU - H4、BIU - H6、BIU - H9，该抗体被小鼠抗人淋巴细胞血清阻断。通过ABC - ELISA和IIF检测针对各种细胞详细描述了它们的反应模式。它们与膀胱移行细胞癌呈阳性反应，与正常膀胱呈阴性反应。BIU - H6与任何其他正常或恶性组织均无反应。BIU - H4、6的免疫球蛋白亚类为IgG1，BIU - H9的为IgG2α。BIU - H6、9识别的抗原分子量为102 KD。
