Kaartinen N, Kananen K, Huhtala H, Keränen S, Tinkanen H
Department of Obstetrics and Gynaecology, Tampere University Hospital, University of Tampere, Teiskontie 35, 33521, Tampere, Finland.
Infertility Clinic Ovumia, Biokatu 12, 33520, Tampere, Finland.
J Assist Reprod Genet. 2016 Mar;33(3):393-399. doi: 10.1007/s10815-015-0642-8. Epub 2016 Jan 9.
The aim of this study was to study the effect of the embryo freezing method on the birth weight of newborns from frozen embryo transfer (FET) cycles, and the pregnancy results of cleavage stage embryos cryopreserved by slow freezing or vitrification.
This is a retrospective cohort study undertaken in a University Hospital IVF unit using concurrently both the slow-freezing and the vitrification techniques. All frozen-thawed and vitrified-warmed day 2 and day 3 embryo transfers during the time period from 1 April 2009 to 31 November 2013 were included in the study.
There was no statistically significant weight difference between newborns from vitrified or slow-frozen embryos (3588 vs 3670 g). A higher post-thaw viability rate was achieved after cryopreservation by the vitrification technique compared to the slow-freezing protocol (83.4 vs 61.4%). The miscarriage rate was lower in the vitrification group (15.7 vs 29.0%). The live birth rates were similar (19.5 vs 19.1%) in the slow-freezing and vitrification groups, respectively. Among vitrified embryos, 7.4 embryos needed to be thawed to produce one delivery; in the slow-freezing group, that number was 11.9.
The freezing method has no impact on the weight of the newborn. With lower post-thaw survival rates and higher miscarriage rates, the slow-freezing cryopreservation protocol is inferior to the vitrification technique.
本研究旨在探讨胚胎冷冻方法对冻融胚胎移植（FET）周期新生儿出生体重的影响，以及慢速冷冻或玻璃化冷冻的卵裂期胚胎的妊娠结局。
这是一项在大学医院体外受精科进行的回顾性队列研究，同时使用慢速冷冻和玻璃化技术。纳入2009年4月1日至2013年11月31日期间所有冻融及玻璃化复温的第2天和第3天胚胎移植。
玻璃化冷冻或慢速冷冻胚胎的新生儿体重无统计学显著差异（3588对3670克）。与慢速冷冻方案相比，玻璃化技术冷冻保存后的解冻后存活率更高（83.4%对61.4%）。玻璃化组的流产率更低（15.7%对29.0%）。慢速冷冻组和玻璃化组的活产率相似（分别为19.5%和19.1%）。在玻璃化冷冻胚胎中，每出生一例需要解冻7.4枚胚胎；在慢速冷冻组，这一数字为11.9枚。
冷冻方法对新生儿体重无影响。慢速冷冻保存方案解冻后存活率较低，流产率较高，不如玻璃化技术。
