Singh A K, Jain A, Jain B, Singh K P, Dangi T, Mohan M, Dwivedi M, Kumar R, Kushwaha R A S, Singh J V, Mishra A C, Chhaddha M S
Department of Microbiology, NIV, Infl uenza division, King George's Medical University, Lucknow, Uttar Pradesh, India.
Indian J Med Microbiol. 2014 Jan-Mar;32(1):13-8. doi: 10.4103/0255-0857.124288.
Acute lower respiratory tract infections (ALRI), ranked as the second leading cause of death are the primary cause of hospitalisation in children. Viruses are the most important causative agents of ALRI.
To study the viral aetiology of ALRI in children at a tertiary care hospital.
One year prospective observational study in a tertiary care hospital of King George's Medical University, Lucknow.
Nasopharyngeal aspirate (NPA) was collected from children admitted with signs and symptoms of ALRI who were aged 0-14 years. Samples were transported to the laboratory at 4°C in viral transport media and processed for detection of respiratory syncytial virus (RSV) A and B, influenza virus A and B, adenovirus (ADV), human Boca virus (HBoV), human metapneumo virus (hMPV) and parainfluenzavirus 1, 2, 3 and 4 using mono/multiplex real-time polymerase chain reaction (RT-PCR). STATA was used for statistical analysis.
In one year, 188 NPAs were screened for respiratory viruses, of which 45.7% tested positive. RSV was most commonly detected with 21.3% positivity followed by measles virus (8.5%), influenza A virus (7.4%), ADV (5.3%), influenza B virus (1.6%), hMPV (1.1%) and HBoV (0.5%). Month wise maximum positivity was seen in December and January. Positivity rate of RSV was highest in children aged < 1 year, which decreased with increase in age, while positive rate of influenza virus increased with increasing age.
The occurrence of viral predominance in ALRI is highlighted.
急性下呼吸道感染（ALRI）是儿童住院的主要原因，在死亡原因中排名第二。病毒是ALRI最重要的病原体。
研究一家三级护理医院中儿童ALRI的病毒病因。
在勒克瑙乔治国王医科大学的一家三级护理医院进行为期一年的前瞻性观察研究。
从0至14岁有ALRI体征和症状的住院儿童中采集鼻咽抽吸物（NPA）。样本在4°C下于病毒运输培养基中运至实验室，并使用单重/多重实时聚合酶链反应（RT-PCR）检测呼吸道合胞病毒（RSV）A和B、甲型和乙型流感病毒、腺病毒（ADV）、人博卡病毒（HBoV）、人偏肺病毒（hMPV）以及副流感病毒1、2、3和4。使用STATA进行统计分析。
一年内，对188份NPA进行了呼吸道病毒筛查，其中45.7%检测呈阳性。最常检测到的是RSV，阳性率为21.3%，其次是麻疹病毒（8.5%）、甲型流感病毒（7.4%）、ADV（5.3%）、乙型流感病毒（1.6%）、hMPV（1.1%）和HBoV（0.5%）。12月和1月的月度阳性率最高。RSV的阳性率在<1岁儿童中最高，随年龄增长而降低，而流感病毒的阳性率随年龄增加而升高。
强调了ALRI中病毒占优势的情况。
