Wiebenga Oliver T, Klauser Antoine M, Nagtegaal Gijsbert J A, Schoonheim Menno M, Barkhof Frederik, Geurts Jeroen J G, Pouwels Petra J W
Department of Radiology and Nuclear Medicine, Neuroscience Campus Amsterdam and VU University Medical Center, Amsterdam, the Netherlands; Department of Anatomy and Neurosciences, Neuroscience Campus Amsterdam and VU University Medical Center, Amsterdam, the Netherlands.
NMR Biomed. 2014 Mar;27(3):304-11. doi: 10.1002/nbm.3063. Epub 2014 Jan 7.
The purpose of this study was to evaluate quality parameters, metabolite concentrations and concentration ratios, and to investigate the reproducibility of quantitative proton magnetic resonance spectroscopic imaging ((1)H-MRSI) of selected white and gray matter regions of healthy adults. 2D-quantitative short-TE (1)H-MRSI spectra were obtained at 1.5T from the healthy human brain. Subjects (n = 12) were scanned twice with an interval of six months. Absolute metabolite concentrations were obtained based on coil loading, taking into account differences in sensitivity of the phased-array head coil. Spectral quality parameters, absolute metabolite concentrations, concentration ratios, and their reproducibility were determined and compared between time-points using a repeated measures general linear model. The quality of the spectra of selected brain areas was good, as determined by a mean spectral linewidth between 4.8 and 7.3 Hz (depending on the region). No significant differences between the two time-points were observed for spectral quality, concentrations, or concentration ratios. The mean intrasubject coefficient of variation (CoV) varied between 4.0 and 8.5% for total N-acetylaspartate, 7.2 and 10.8% for total creatine, 5.9 and 9.8% for myo-inositol, and 8.0 and 13.3% for choline, and remained below 20% for glutamate. CoV was generally lower when concentration ratios were considered. The study shows that longitudinal quantitative short-TE (1)H-MRSI generates reproducible absolute metabolite concentrations in healthy human white and gray matter. This may serve as a background for longitudinal clinical studies in adult patients.
本研究的目的是评估质量参数、代谢物浓度和浓度比，并研究健康成年人选定白质和灰质区域的定量质子磁共振波谱成像（(1)H-MRSI）的可重复性。在1.5T条件下从健康人脑获取二维定量短回波时间（(1)H-MRSI）谱。受试者（n = 12）间隔六个月进行两次扫描。基于线圈负载获取绝对代谢物浓度，同时考虑相控阵头线圈灵敏度的差异。使用重复测量通用线性模型确定并比较时间点之间的光谱质量参数、绝对代谢物浓度、浓度比及其可重复性。所选脑区的光谱质量良好，平均谱线宽度在4.8至7.3Hz之间（取决于区域）。在光谱质量、浓度或浓度比方面，两个时间点之间未观察到显著差异。总N-乙酰天门冬氨酸的受试者内变异系数（CoV）平均值在4.0%至8.5%之间，总肌酸为7.2%至10.8%，肌醇为5.9%至9.8%，胆碱为8.0%至13.3%，谷氨酸低于20%。考虑浓度比时，CoV通常较低。该研究表明，纵向定量短回波时间（(1)H-MRSI）可在健康人白质和灰质中生成可重复的绝对代谢物浓度。这可为成年患者的纵向临床研究提供背景。
