雌激素治疗会使糖尿病小鼠易患严重且持续性的阴道念珠菌病。

Estrogen treatment predisposes to severe and persistent vaginal candidiasis in diabetic mice.

作者信息

Hamad Mawieh

机构信息

Department of Medical Laboratory Sciences, College of Health Sciences, University of Sharjah, PO Box 27272, Sharjah, UAE.

出版信息

J Diabetes Metab Disord. 2014 Jan 8;13(1):15. doi: 10.1186/2251-6581-13-15.

DOI:10.1186/2251-6581-13-15

PMID:24401317

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3916058/

Abstract

BACKGROUND

Increased levels of estrogen and diabetes mellitus separately predispose to vaginal candidiasis (VC). However, the compounding effect of estrogen on the severity and persistence of VC in diabetic females is not clear.

METHODS

To address this issue, a diabetic mouse model with estrogen-maintained VC was developed and evaluated for vaginal fungal burden (VFB) and immune competence at different time points throughout the study period.

RESULTS

Blood glucose levels in estrogen-treated diabetic mice were consistently lower than that in untreated counterparts. Estrogen-treated C. albicans-infected non-diabetic mice experienced persistent episodes of VC as compared with naïve controls (P < 0.01). However, severity and persistence of VC in estrogen-treated C. albicans-infected diabetic mice was significantly greater than that in non-diabetic counterparts (P < 0.05). Mortality rates among estrogen-treated C. albicans-infected diabetic mice were significantly higher (P < 0.05) than that in non-diabetic counterparts. Statistically significant (P < 0.05) and persistent suppression of the delayed hypersensitivity response (DTH) was evident in estrogen-treated C. albicans-infected diabetic and non-diabetic mice as compared with controls. Levels of expression of the inhibitory molecule CD152 on vaginal and splenic T cells isolated from estrogen-treated C. albicans infected mice was significantly higher than that in naive untreated controls (P < 0.01).

CONCLUSIONS

These findings suggest that estrogen treatment in diabetic females may protect against the progression of DM on the one hand and predispose to severe and persistent VC on the other. The later outcome could be related to the immunosuppressed status of the host.

摘要

背景

雌激素水平升高和糖尿病分别易引发阴道念珠菌病（VC）。然而，雌激素对糖尿病女性VC严重程度和持续性的复合影响尚不清楚。

方法

为解决这一问题，构建了雌激素维持的VC糖尿病小鼠模型，并在整个研究期间的不同时间点评估其阴道真菌负荷（VFB）和免疫能力。

结果

雌激素处理的糖尿病小鼠的血糖水平始终低于未处理的对照小鼠。与未感染的对照相比，雌激素处理的白色念珠菌感染的非糖尿病小鼠经历了持续性的VC发作（P < 0.01）。然而，雌激素处理的白色念珠菌感染的糖尿病小鼠中VC的严重程度和持续性明显高于非糖尿病对照小鼠（P < 0.05）。雌激素处理的白色念珠菌感染的糖尿病小鼠的死亡率明显高于非糖尿病对照小鼠（P < 0.05）。与对照相比，雌激素处理的白色念珠菌感染的糖尿病和非糖尿病小鼠中，迟发型超敏反应（DTH）受到统计学显著（P < 0.05）且持续的抑制。从雌激素处理的白色念珠菌感染小鼠中分离出的阴道和脾脏T细胞上抑制分子CD152的表达水平明显高于未处理的未感染对照（P < 0.01）。