Department of Microbiology and Immunology, RMSB 3035, University of Miami Miller School of Medicine, 1600 NW 10th Avenue, Miami, FL 33136, USA.
Clin Exp Immunol. 2012 Nov;170(2):131-8. doi: 10.1111/j.1365-2249.2012.04650.x.
The mRNA levels of a set of immune-related genes were analysed with peripheral blood samples from at-risk, new-onset and long-term type 1 diabetes (T1D) patients, in comparison to those from healthy controls. The selected set includes T lymphocyte genes [CD3G and cytotoxic T lymphocyte-associated antigen 4 (CTLA4)], B lymphocyte genes (CD19 and CD20) and myeloid cell-related genes [CD11b, Toll-like receptor (TLR)-9, arginase (ARG1)]. Also included is a subset of the S100 family members that has been documented recently as regulatory elements of innate immunity. Samples from patients with long-term T1D had a reduced level of mRNA for most of selected innate and adaptive immune genes. No such reduction was detected in samples collected from at-risk or new-onset T1D patients. Analyses of regulatory gene expression ratios revealed a dynamic disproportion of CTLA4 versus CD3G expression in samples from at-risk, new-onset and long-term T1D patients. These changes could serve as immunological biomarkers for the status of the immune system during T1D progression and therapeutic interventions.
采用外周血样本分析了一组与免疫相关的基因在处于危险中、新发病和长期 1 型糖尿病（T1D）患者中的 mRNA 水平，并与健康对照进行了比较。所选基因集包括 T 淋巴细胞基因[CD3G 和细胞毒性 T 淋巴细胞相关抗原 4（CTLA4）]、B 淋巴细胞基因（CD19 和 CD20）和髓样细胞相关基因[CD11b、Toll 样受体（TLR）-9、精氨酸酶（ARG1）]。最近还记录了 S100 家族成员的一个亚群作为先天免疫的调节因子。来自长期 T1D 患者的样本中，大多数选择的先天和适应性免疫基因的 mRNA 水平降低。在处于危险中或新发病 T1D 患者采集的样本中未检测到这种减少。调节基因表达比率的分析显示，在处于危险中、新发病和长期 T1D 患者的样本中，CTLA4 与 CD3G 表达的动态失衡。这些变化可作为 T1D 进展和治疗干预期间免疫系统状态的免疫生物学标志物。
